ZHANG Yu-ping,JIANG Ming,LU Shao-wei,et al.Genetic Relationship Analysis of Blackberries by AFLP[J].Northern Horticulture,2013,37(21):116-119.
黑莓亲缘关系的AFLP分析
- Title:
- Genetic Relationship Analysis of Blackberries by AFLP
- 文章编号:
- 1001-0009(2013)21-0116-04
- Keywords:
- blackberry; genetic relationship; AFLP
- 分类号:
- S 663.2
- 文献标志码:
- A
- 摘要:
- 以引进的11个黑莓栽培品种和13株黑莓实生苗为试材，采用AFLP分子标记技术，从EcoRⅠ/MseⅠ引物组合中筛选出10对多态性好、扩增条带清晰的引物对其进行遗传多样性和亲缘关系的研究。结果表明：黑莓样品共扩增出657条带，多态性条带488条，多态性比率为74.3%。基于品种间遗传相似性系数的UPGMA聚类分析，在遗传相似性系数0.52水平上可将黑莓分为2组，与形态学分类基本一致。表明AFLP技术可有效应用于黑莓品种遗传关系的研究，为品种鉴定、合理引种以及遗传育种提供可靠依据。
- Abstract:
- Taking 11 blackberry cultivars introduced abroad and 13 excellent seedlings of blackberry as materials，a total of 10 primer pairs selected from EcoRⅠ/MseⅠ primer combinations were used to analysis the genetic diversity and genetic relationship of them by AFLP.The results indicated that it produced 657 AFLP-bands，488 of which were polymorphic bands，the polymorphic rate was 74.3%.The cluster analysis based on UPGMA showed that the 24 genotypes were classified into 2 groups at the level of SM=0.52，this result was coincidence with the morphological classification.It concluded that AFLP was an effective way to study of genetic diversity among blackberries accessions and will be helpful in identifying varieties，introduction reasonably and breeding purposes as well as further research.
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备注/Memo
第一作者简介：张玉平（1972-），女，硕士，副研究员，现主要从事树莓遗传育种等研究工作。