WANG Li-juan,SHI Jing,WANG Yan-cai.Isolation and Expression Analysis of Sucrose Synthase Gene cDNA from Lycium barbarum L.[J].Northern Horticulture,2013,37(21):105-109.
枸杞蔗糖合成酶基因cDNA分离及表达分析
- Title:
- Isolation and Expression Analysis of Sucrose Synthase Gene cDNA from Lycium barbarum L.
- 文章编号:
- 1001-0009(2013)21-0105-05
- 分类号:
- S 567.1+9
- 文献标志码:
- A
- 摘要:
- 以“宁杞1号”为试材，根据已知植物物种蔗糖合成酶基因（Susy）保守区序列设计引物，进行Lb Susy基因保守区的克隆，并根据保守片段核苷酸序列，利用RACE技术扩增基因全长。然后分析Lb Susy基因生物学信息，同时利用Real-time PCR检测Susy基因在枸杞不同组织器官中的表达量。结果表明：经RT-PCR扩增得到长度为2 926 bp的基因片段，克隆到pGM-T Easy载体中，命名为Lb Susy（GenBank：KC907702）。推导氨基酸序列与番茄、欧洲桤木等蔗糖合成酶基因氨基酸序列同源性为100%～84%。运用PSORT服务器亚细胞定位分析表明，Lb Susy编码的蔗糖合成酶蛋白质定位于线粒体。Real-time PCR表达分析显示，Lb Susy基因在枸杞茎中表达量最高，在根中表达水平较低。
- Abstract:
- Taking ‘Ningqi No.1’ as material，the primers were designed according to the conserved domains of the plant sucrose synthase genes’ nucleotide sequences，and a sucrose synthase gene was cloned from Lycium barbarum L.by RT-PCR method.The full length of Susy gene was cloned by RACE.Its biological information was analyzed and gene expression levels in different organs were tested using Real-time PCR.The results showed that the fragment cloned named Lb Susy (GenBank number：KC907702) was 2 926 bp in length.The amino acid sequences were 84%～100% identical to the sequences of tomato，Alnus glutinosa ?etc.The putative protein encoded by Lb Susy was gone to mitochondrion by PSORT analysis software.Real time PCR analysis showed that the Lb Susy gene was highly expressed in stem.The expression level reached the lowest level in root.
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备注/Memo
第一作者简介：王丽娟（1974-），女，博士，副教授，现主要从事植物基因工程研究工作。E-mail:Lijuanwang279@hotmail.com.