WEN Gang,LIU Hai-yan,YANG Mei.Optimization of Liquid Culture Medium of Armillaria mellea Exopolysaccharides[J].Northern Horticulture,2013,37(19):152-154.
蜜环菌胞外多糖液体发酵培养基的优化
- Title:
- Optimization of Liquid Culture Medium of Armillaria mellea Exopolysaccharides
- 文章编号:
- 1001-0009(2013)19-0152-03
- Keywords:
- Armillaria mellea; exopolysaccharides; culture medium; orthogonal test
- 分类号:
- S 646
- 文献标志码:
- A
- 摘要:
- 以蜜环菌为试材，研究了碳源、氮源、无机离子等因素对胞外多糖产量的影响，并利用正交实验对蜜环菌胞外多糖液体发酵培养基进行了优化研究。结果表明：适宜胞外多糖生产的培养基组分为：蔗糖25.00 g/L，豆饼粉2.50 g/L，K2HPO4 1.50 g/L，KH2PO4 0.25 g/L，MgSO4 0.50 g/L，ZnSO4 0.03 g/L，FeSO4 0.02 g/L。利用此培养基发酵生产胞外多糖产量可达478.5±22.4 mg/L，是基本培养基的6倍。
- Abstract:
- Taking Armillaria mellea as material,the effect of carbon source，nitrogen source and inorganic ion on the yield of exopolysaccharides were studied，and the liquid culture medium of it was optimized by orthogonal test.The results showed that the optimal culture medium was：sucrose 25.00 g/L，peptone 2.50 g/L，MgSO4 0.50 g/L，K2HPO4 1.50 g/L，KH2PO4 0.25 g/L，ZnSO4 0.03 g/L，and FeSO4 0.02 g/L.In these conditions，the exopolysaccharides of Armillaria mellea can reach 478.5±22.4 mg/L，it was 6 times as the mycelium biomass of the basal medium.
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备注/Memo
第一作者简介：温钢（1976-），男，吉林四平人，硕士，讲师，研究方向生物活性物质开发。