[1]蒋素华,田云芳,黄 萍,等.紫萼的组培快繁技术研究[J].北方园艺,2013,37(18):94-96.
　JIANG Su-hua,TIAN Yun-fang,HUANG Ping,et al.Study on Tissue Culture and Rapid Propagation of Hosta ventricosa [J].Northern Horticulture,2013,37(18):94-96.
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紫萼的组培快繁技术研究

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第37卷 期数: 2013年18期 页码: 94-96 栏目: 出版日期: 2013-09-30
Title:
Study on Tissue Culture and Rapid Propagation of Hosta ventricosa
文章编号:
1001-0009(2013)18-0094-03
作者:
蒋素华1; 田云芳1; 黄 萍2; 李艳辉3; 崔 波1
1.郑州师范学院，河南 郑州 450004；
2.河南农业职业学院，河南 郑州 451450；
3.封丘县农业局，河南 新乡 453300
Author(s):
JIANG Su-hua1; TIAN Yun-fang1; HUANG Ping2; LI Yan-hui3; CUI Bo1
1.Zhengzhou Normal University,Zhengzhou,Henan 450004;
2.Henan Vocational College of Agriculture,Zhengzhou,Henan 451450;
3.Fengqiu Bureau of Agriculture,Xinxiang,Henan 453300
关键词:
紫萼; 快速繁殖; 组织培养
Keywords:
Hosta ventricosa; rapid propagation; tissue culture
分类号:
S 682.1+9
文献标志码:
B
摘要:
以紫萼幼苗为试材，对紫萼的组培快繁技术进行了研究，以期建立紫萼的再生体系。结果表明：紫萼的最适愈伤组织诱导分化培养基为MS+6-BA 1.0 mg/L+IBA 0.5 mg/L+蔗糖3%+琼脂9.0 mg/L；最适增殖培养基为MS+6-BA 1.0 mg/L+NAA 0.1 mg/L+蔗糖3%+琼脂9.0 mg/L；最适生根培养基为1/2MS+NAA 0.5 mg/L+蔗糖3%+琼脂9.0 mg/L。
Abstract:
Taking the seedlings of Hosta ventricosa as material,the regeneration system of Hosta ventricosa was established,and the rapid propagation technology was studied.The results showed that the most appropriate medium for callus induction was MS+6-BA 1.0 mg/L+IBA 0.5 mg/L+sugar 3%+agar 9.0 mg/L;the best medium for proliferation was MS+6-BA 1.0 mg/L+NAA 0.1 mg/L+sugar 3%+agar 9.0 mg/L;the best medium for rooting was 1/2MS+NAA 0.5 mg/L+sugar 3%+agar 9.0 mg/L.

参考文献/References:

[1]   张金政,施爱萍,孙国峰,等.玉簪属植物研究发展[J].园艺学报,2004,31(4):550-552.
[2]   李书心.辽宁植物志(下册)[M].沈阳:辽宁科学技术出版社,1991:684-685.
[3]   韩全忠,王正兴.大连地区植物志(下册)[M].大连:大连理工大学出版社,1993:966-967.
[4]   Paul A,Canon L E,John E.The Hosta Book[M].London:BT Barsford Ltd,1992:104-118.
[5]   虞耀瑾,王泰哲.玉簪组织培养器官的建成[J].上海农业学报,1996,12(1):32-35.
[6]   南京中医药大学.中药大辞典(下册)[M].上海:上海科学技术出版社,2006:3283.
[7]   冯慧,王茂亮,王建红,等.玉簪花器官植株再生体系的建立[J].北京农学院学报,2006,21(3):42-45.
[8]   周青,任旭琴.玉簪的组培快繁技术研究[J].江苏农业科学,2005(6):89-91.
[9]   吴延军.香石竹茎尖脱毒技术[J].西南园艺,2003,31(1):20.
[10] 崔澄.植物激素与细胞分化及形态发生的关系[J].细胞生物学,1983(5):1-6.
[11] 崔丽华.植物生长调节物质对组织培养中不定芽不定根的作用[J].辽宁师范专科学校学报,2000(2):97-99.
[12] 李钱鱼,夏宜平.花叶玉簪优新品种及其繁殖技术[J].中国花卉园艺,2003(19):31-33.
[13] Papachatzi M,Hammer P A,Hasegawa P M.In vitro propagation of Hosta plantaginea[J].Hort Science,1980,15(4):506-507.
[14] Papachatzi M,Hammer P A,Hasegawa P M.In vitro propagation of Hosta decorat ‘Thomas Hogg’ using cultured shoot tips[J].J Am Soc Hort Sci,1981,106(2):232-236.
[15] 张洪娣,张琛,吕卉,等.紫萼快速繁殖的研究[J].湖北农业科学,2011,50(1):180-182.
[16] 王春婷,石大兴,王米力.紫萼玉簪的组织培养和快速繁殖[J].植物生理学通讯,2006,42(4):685.

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备注/Memo

第一作者简介：蒋素华（1983-），女，河南漯河人，硕士，讲师，现主要从事花卉分子生物学研究工作。
责任作者：崔波（1962-），男，河南驻马店人，博士，研究员，现主要从事花卉分子生物学研究工作。
基金项目：郑州市科技攻关资助项目（074SGYS33205-2）；
               郑州市重点科技攻关资助项目（051SGDS17017）。
收稿日期：2013-04-15

更新日期/Last Update: 2014-08-15