ZHENG Ke,DONG Sheng-jun,TIAN Ming-fang,et al.Study on Tissue Culture Proportion Technology of Prunus sibirica[J].Northern Horticulture,2013,37(03):115-118.
山杏组培繁殖技术研究
- Title:
- Study on Tissue Culture Proportion Technology of Prunus sibirica
- 文章编号:
- 1001-0009(2013)03-0115-04
- Keywords:
- Prunus sibirica; tissue culture; proportion technology
- 分类号:
- S 662.204+.3
- 文献标志码:
- A
- 摘要:
- 以山杏为试材，研究了外植体的消毒、基本培养基筛选、外植体的种类及不同浓度配比的激素对外植体各阶段生长的影响。结果表明：采用1.0 g/L的HgCl2消毒3 min灭菌效果最好；以改良MS[C(NH4+)∶C(NO3-)=0.16]为基本培养基，组培苗分化和生长效果较好；最适外植体为茎尖；初代培养最适的激素配比为1.0 mg/L的6-BA和0.05 mg/L的NAA；继代培养最适的激素配比为0.5 mg/L的6-BA和0.1 mg/L的NAA。
- Abstract:
- The Prunus sibirica was used as material，the kind of basic medium，the kind of explants and the different proportions of hormones impact the growth condition of explants in the various stages of tissue culture were studied.The results showed that the best sterilization effect was used HgCl2 1 g/L as disinfectants to disinfect 3 min；the division and growth condition of explants was better in improved MS[C(NH4+)∶C(NO3-)=0.16]medium；the most suitable explants was shoot tips；the most suitable proportions of hormones was 6-BA 1.0 mg/L and NAA 0.05 mg/L in primary culture；the most suitable proportions of hormones was 6-BA 0.5 mg/L and NAA 0.1 mg/L in subculture.
参考文献/References:
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备注/Memo
第一作者简介：郑可（1987-），女，硕士，现主要从事森林培育等方面的研究工作。E-mail:zhengke19870430@163.com.