YANG Rui-hong,SHI Chong,WANG Chun-li.Study on the Isolation and Degradation Spectrum of Organophosphorus-degradtion Bacteria in Pear Orchard of Southern Xinjiang[J].Northern Horticulture,2013,37(24):123-126.
南疆梨园有机磷降解菌的分离及其降解谱研究
- Title:
- Study on the Isolation and Degradation Spectrum of Organophosphorus-degradtion Bacteria in Pear Orchard of Southern Xinjiang
- 文章编号:
- 1001-0009(2013)24-0123-04
- 分类号:
- S 661.2（245）
- 文献标志码:
- A
- 摘要:
- 以新疆南疆长期使用有机磷农药的果园土壤为研究对象，分离其中能够完全降解氧化乐果有效成分的细菌，利用16S rDNA对其进行分子鉴定，并对其降解有机磷农药的降解谱和降解能力进行了分析。结果表明：试验共分离得到3株能够完全降解氧化乐果有效成分的细菌，这3株菌株分别为假单孢菌、芽孢杆菌和土壤杆菌；不同菌株对有机磷农药的降解谱存在差异，其中假单孢菌DYY-3的降解能力较其它2株菌高。该研究结果为今后关于菌株复配制剂及其田间应用奠定了良好的基础。
- Abstract:
- Taking the pear orchards soils in Southern Xinjiang as research object,bacteria strains were isolated from them which could degrade the effective components of omethoate completely.The degrading strains were identified using 16S rDNA sequences.The degradation spectrum and degradation capabilities of them were studied.The results showed that 3 strains were isolated which could degrade the effective components of omethoate completely，and they were identified as Pseudomonadales sp.，Bacillus spp.and Agrobacterium sp.There were differences among different strains.Of the three bacteria strains，the strain of Pseudomonas sp.DYY-3 exhibited higher degradation capabilities.The data of this study provided the solid foundation for microbial-insecticide mixtures and its field application.
参考文献/References:
[1]付广云,韩长秀.有机磷农药及其危害[J].化学教育,2005(1):9-10.
[2]Singh B K.Organophosphorus-degrading bacteria:ecology and industrial applications[J].Nat Rev Microbiol,2009(7):156-164.
[3]Singh B K，Walker A.Microbial degradation of organophosphorus compounds[J].FEMS Microbiol Rev,2006（30）:428-471.
[4]孔繁翔,严国安,尹大强,等.环境生物学[M].北京:高等教育出版社,2000.
[5]王一惠,向述荣,张银定,等.微生物降解苯酚的研究进展及其工程菌的应用前景[J].高技术通信,2001,12(4):98-100.
[6]余水静,李杰庆,宋秋华,等.两株苯酚降解菌的分离及降解特性的初步研究[J].生物技术,2005,15(3):62-64.
[7]徐玉泉,张维,陈明,等.一株苯酚降解的分离和鉴定[J].环境科学导报,2000,20(4):450-455.
[8]龚斌,刘津,赵斌,等.一株高效苯酚降解菌的分离、鉴定及降解特性的研究[J].环境科学学报,2006(12):2008-2012.
[9]李淑彬,钟英长.固定化假单胞菌降解甲胺磷的研究[J].应用与环境生物学报,1999,5(4):422-466.
[10]赫荣乔.探索假单胞菌降解4-氯硝基苯代谢新途径[J].微生物学通报,2008,35(3):363.
[11]孙纪全,汤岳琴,刘伟强,等.两株假单胞菌降解酚类化合物的特性[J].中国环境科学,2010,30(12):1633-1638[12]杜春梅,金术超,范晶,等.侧孢芽孢杆菌降解水胺硫磷的条件及途径[J].北方园艺,2008(11):170-173.
[13]宫占元,王艳杰,李永鹏,等.侧孢芽孢杆菌降解有机磷能力的研究[J].黑龙江八一农垦大学学报,2005,17(5):14-17.
[14]宫占元,王艳杰,李永鹏,等.侧孢芽孢杆菌降解无机磷能力的研究[J].黑龙江八一农垦大学学报,2006,18(1):12-14.
[15]仉磊,袁红莉,汪双清,等.一株土壤杆菌降解菲的代谢途径初探[J].中国科学（地球科学）,2005,35(增Ⅰ):226-232.
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备注/Memo
第一作者简介：杨瑞红（1980-），女，博士研究生，讲师，现主要从事生物学等基础教育研究工作。E-mail:yangruihong99@163.com.
责任作者：王纯利（1959-）,男，副教授，现主要从事环境微生物研究工作。E-mail:wangchunli;59@yahoo.com.cn.
基金项目：国家重点学科博士基金资助项目（XJCYB-2011-01）；新疆草地资源与生态自治区重点实验室对外开放课题资助项目（XJDX0209-2011-02）。
收稿日期：2013-04-15