YANG Rui-hong,SONG Bin,LU Yun,et al.Isolation and Degraded Effect of DDVP-degrading Bacteria in Soil of Pear Orchards[J].Northern Horticulture,2014,38(20):164-168.
梨园土壤中敌敌畏降解菌的分离及其降解效能比较
- Title:
- Isolation and Degraded Effect of DDVP-degrading Bacteria in Soil of Pear Orchards
- 文章编号:
- 1001-0009（2014）20-0164-05
- Keywords:
- pear orchard; soil; biodegradation; DDVP; soil bacteria
- 分类号:
- S 661.206+.1
- 文献标志码:
- A
- 摘要:
- 从新疆南疆长期使用有机磷农药的梨园土壤中分离出18株能够降解敌敌畏有效成分DDVP的菌株。采用驯化培养，形态观察及16S rDNA测序后，利用NCBI数据库Blast比对鉴定。结果表明：所分离的菌株有12株假单胞菌、4株芽孢杆菌、1株微杆菌和1株土壤杆菌，其中以假单胞菌为主；所获得的菌株对敌敌畏制剂的降解能力普遍低于DDVP纯品；混合菌株能够明显的提高降解敌敌畏制剂的效果；混合降解后菌液中的菌株比例发生了较大的改变，但假单胞菌仍然为其中的优势菌株；该研究为进一步研究其降解机制奠定良好的基础。
- Abstract:
- 18 strains of bacteria were isolated from the pear orchards soils in southern Xinjiang，which could degrade DDVP (effective constituent of Dichlorvos agent) habituated culture and morphologic observation were adopted,and blasted 16S rDNA sequence in NCBI database.The results showed that those strains were identified as 4 groups，14 strains werePseudomonadales sp.，4 strains were Microbacterium sp.，1 strain was Bacillus spp.and 1 strain was Agrobacterium sp., Pseudomonadales sp.was dominant group.In degraded DDVP experiments，compared with pure DDVP，the degradation effect of dichlorvos was lower.Mixture the 4 strains with same proportion could enhance the degradation effect of dichlorvos，which may be due to every strains’ different roles in the complex degrading process of organic phosphorus.After the dichlorvos degradation，the percentage of each strain of mixture was significantly changed.Pseudomonadales sp.was still dominant species.This study would provide strong support for further study of degraded mechanism.
参考文献/References:
[1]付广云,韩长秀.有机磷农药及其危害[J].化学教育,2005(1):9-10.
[2]Singh B K.Organophosphorus-degrading bacteria:ecology and industrial applications[J].Nat Rev Microbiol,2009(7):156-164.
[3]Singh B K,Walker A.Microbial degradation of organophosphorus compounds[J].FEMS Microbiol Rev,2006,30(3):428-471.
[4]周波,鲍长利,冯志兵,等.天然沸石负载TiO2光催化降解敌敌畏和对硫磷[J].环境污染治理技术与设备,2004,5(6):33-35.
[5]刘春英,弓晓峰,张政辉.光催化氧化法降解有机磷农药的研究[J].四川环境,2006,25(6)：5-8.
[6]Zhang X H，Zhang G S,Zhang Z H,et al.Isolation and Characterization of a Dichlorvos-Degrading Strain DDV-1 of Ochrobactrum sp.[J].Pedosphere,2006,16(1):64-67.
[7]王俊,张干,李军,等.富产敌敌畏降解酶菌株的筛选及降解性能研究[J].环境与健康杂志,2006,23(4):310-312. [8]李荣,贾开志,蒋建东,等.敌敌畏、敌百虫高效降解菌株DDB-1的分离鉴定及降解特性研[J].农业环境科学学报,2007,26(2):554-558.
[9]蔡颖慧,张惠文,苏振成,等.敌敌畏降解菌的分离鉴定及降解特性研究[J].生物技术,2009,19(2):60-62.
[10]杨瑞红,施宠,王纯利.南疆梨园有机磷降解菌的分离及其降解谱研究[J].北方园艺,2013(24):123-126.
[11]李淑彬,钟英长.固定化假单胞菌降解甲胺磷的研究[J].应用与环境生物学报，1999,5(4):422-466.
[12]赫荣乔.探索假单胞菌降解4-氯硝基苯代谢新途径[J].微生物学通报,2008,35(3):363.
[13]孙纪全,汤岳琴,刘伟强,等.两株假单胞菌降解酚类化合物的特性[J].中国环境科学，2010,30(12):1633-1638.
[14]杜春梅,金术超,范晶,等.侧孢芽孢杆菌降解水胺硫磷的条件及途径[J].北方园艺,2008(11)：170-173.
[15]宫占元,王艳杰,李永鹏,等.侧孢芽孢杆菌降解有机磷能力的研究[J].黑龙江八一农垦大学学报,2005,17(5):14-17.
[16]宫占元,王艳杰,李永鹏,等.侧孢芽孢杆菌降解无机磷能力的研究[J].黑龙江八一农垦大学学报,2006,18(1):12-14.
[17]Cabrera J A,Andreas K,Richard A,et al.Alexander SchoutenIsolation and characterization of fenamiphos degrading bacteria[J].Biodegradation,2010(21):1017-1027.
[18]Ca′ceres T P,Megharaj M,Malik S,et al.Hydrolysis of fenamiphos and its toxic oxidation productsby Microbacterium sp.in pure culture and groundwater[J].Bioresour Technol,2009(100):2732-2736.
[19]Arrault S,Desaint S,Catroux C,et al.Isolation and characterization of efficient isoxaben-transforming Microbacterium sp.strains from four European soils[J].Pest Manag Sci,2002(58):1229-1235.
[20]Karpouzas D G,Morgan J A,Walker A.Isolation and characterization of 23 carbofuran- degrading bacteria from soils from distant geographical areas[J].Lett Appl Microbiol,2000(31)：353-358.
[21]仉磊,袁红莉,汪双清,等.一株土壤杆菌降解菲的代谢途径初探[J].中国科学D辑,2005,35(增Ⅰ)：226-232.
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备注/Memo
第一作者简介：杨瑞红（1980-），女，新疆和静人，博士研究生，讲师，现主要从事生物学和科学基础教育等研究工作。E-mail：yangruihong99@163.com.
责任作者：王纯利（1959-），男，硕士，副教授，现主要从事环境微生物等研究工作。E-mail：wangchunli59@yahoo.com.cn.
基金项目：新疆自治区自然科学青年基金资助项目（2014211B014）；新疆教育学院院级课题资助项目（XJJY201326）。
收稿日期：2014-06-11