[1]董盼盼,赵美爱,管 聪,等.番茄抗黄化曲叶病毒基因Ty-3的SCAR标记及应用[J].北方园艺,2013,37(23):119-122.
　DONG Pan-pan,ZHAO Mei-ai,GUAN Cong,et al.SCAR Marker and Application of Resistant Gene Ty-3 of Tomato Yellowing Leaf Virus[J].Northern Horticulture,2013,37(23):119-122.
点击复制

番茄抗黄化曲叶病毒基因Ty-3的SCAR标记及应用

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第37卷 期数: 2013年23期 页码: 119-122 栏目: 出版日期: 2013-12-15
Title:
SCAR Marker and Application of Resistant Gene Ty-3 of Tomato Yellowing Leaf Virus
文章编号:
1001-0009(2013)23-0119-04
作者:
董盼盼1; 赵美爱2; 3; 4; 管 聪2; 王 富1; 王 辉1
1.青岛农业大学 园艺学院，山东 青岛 266109
2.青岛农业大学 生命科学学院，山东 青岛 266109
3.青岛市主要农作物创新与应用重点实验室，山东 青岛 266109；4.山东省高校植物生物技术重点实验室，山东 青岛 266109
Author(s):
DONG Pan-pan1；ZHAO Mei-ai 2; 3; 4；GUAN Cong2；WANG Fu1；WANG Hui1
1.College of Horticulture，Qingdao Agricultural University，Qingdao，Shandong 266109
2.College of Life Sciences，Qingdao Agricultural University，Qingdao，Shandong 266109
3.Qingdao Key Lab of Germplasm Innovation and Application of Major Crops，Qingdao，Shandong 266109
4.Key Lab of Plant Biotechnology in Universities of Shandong，Qingdao，Shandong 266109
关键词:
番茄黄化曲叶病毒; SCAR; Ty-3; 苗期鉴定; 田间鉴定
Keywords:
TYLCV; SCAR; Ty-3; seedling identification; field identification
分类号:
S 641.2
文献标志码:
A
摘要:
以49份番茄材料为试材，利用特异引物对5份抗病纯合番茄材料（基因型Ty-3/Ty-3）和5份感病纯合番茄材料（基因型ty-3/ty-3）进行PCR扩增，同时对25份番茄高代自交系和14]份番茄杂交一代材料进行检测，并用田间自然发病的方法对这些材料的抗性进行验证。结果表明：经PCR扩增，抗病纯合番茄产生了630 bp的片段，而感病纯合材料扩增出320 bp的片段；该标记能够区分抗病材料、感病材料及杂合抗病材料，是与番茄黄化曲叶病毒病（TYLCV）病抗病基因Ty-3紧密连锁的共显性标记；25份番茄高代自交系中，有4份材料基因型是Ty-3/Ty-3，有7份材料基因型是Ty-3/ty-3，14份材料不含抗病基因Ty-3；14份番茄杂交一代材料中，有4份材料基因型是Ty-3/Ty-3，5份材料基因型是Ty-3/ty-3，5份材料不含抗病基因Ty-3；田间试验表明，分子鉴定与田间发病鉴定的吻合度达78.6%以上；在抗番茄黄化曲叶病毒病育种中，可用PCR方法快速筛选亲本抗性材料，提高育种效率。
Abstract:
Taking 49 tomatos as materials，5 resistant homozygous lines (Ty-3/Ty-3)and 5 homozygous susceptible lines (ty-3/ty-3) were amplified by specific primers 25 generations self-lines and 14 F1 hybrids were amplified using this marker，at the same time these lines were well validated in the field by nature infection.The results showed that the resistant homozygous lines produced about 630 bp PCR fragment，the susceptible genotypes could produce fragment about 320 bp.This marker could distinguish resistant and susceptible lines，and it was a co-dominant marker tightly linked to Ty-3 gene.Among 25 generations self-lines，the genotype of 4 generations self-lines were Ty-3/Ty-3，7 were Ty-3/ty-3 and 14 were  ty-3/ty-3.14 F1 hybrids were amplified by Ty-3 primer，the genotype of 4 F1 hybrids were Ty-3/Ty-3,5 were Ty-3/ty-3 and 5 F1 hybrids didn’t contain Ty-3 gene.These lines were well validated in the field by nature infection and the disease incidence was 78.6% coincident with the result of PCR method.In the TYLCV resistance breeding，PCR was a rapid screening method to improve breeding efficiency.

参考文献/References:

[1] 孙作文,杨进绪,张美珍,等.山东省番茄黄化曲叶病毒病的发生及其防治[J].中国蔬菜,2009(21):5-6.
[2] 许爽,褚云霞,张辉,等.多重PCR技术鉴定番茄Ty-2和Ty-3基因及田间验证[J].分子植物育种,2009,7(5):954-958.
[3] 付蓉蓉,刘杨,陈火英.番茄黄化曲叶病的Ty-1和Ty-3抗性基因的PCR鉴定[J].分子植物育种,2011(9):1647-1652.
[4] 韩璀璇,宋建军,王琳珊,等.番茄黄化卷叶病毒病抗病基因Ty-1的CAPS标记建立[J].中国农学通报,2012,28(1):195-200.
[5] 付琬,辛学锐,金郁,等.番茄Ty-1、Ty-2和Ty-3基因的多重PCR技术鉴定[J].中国园艺文摘,2013(4):7-9.
[6] Ji Y，Betteray B，Smeets J，et al.Co-dominant SCAR Marker，P6-25，for Detection of Ty-3，Ty-3a，and Ty-3b introgressions from three Solanum chilense accessions at 25 cM of Chromosome 6 of Begomovirus-Resistant Tomatoes[EB/OL].Proc Tomato Breeders Table，Tampa，FL，USA.roundtable08.ifas.ufl.edu/Schedule.htm.
[7] Rick C M.Biosystematic studies in Lycopersicon and closely related species ofSolanum[M].In：Hawkes J G，Lester R N，Skelding A D.The Biology and Taxoreomy of the Solanaceae,1979:667-668.
[8] 尹贤贵,王小佳,张赟,等.DNA分子标记及其在番茄遗传育种中的应用[J].西南农业大学学报,2004,26(6):663-668.
[9] 余文贵,赵统敏,杨玛丽,等.番茄黄化曲叶病及其抗病育种研究进展[J].江苏农业学报,2009,25(4):925-930.
[10]Ji Y，Schuster D J，Scott J W.Ty-3，a begomovirus resistance locus near the Tomato yellow leaf curl virus resistance locus Ty-1 on chromosome 6 of tomato[J].Molecular Breeding,2007（20）:271-284.

相似文献/References:

[1]杜玉丽,张子君,李海涛,等.番茄黄化曲叶病毒病抗病基因TY-1的PCR检测[J].北方园艺,2012,36(13):136.
　DU Yu-li,ZHANG Zi-jun,LI Hai-tao,et al.CAPS Marker and Detection of Resistant Gene Tomato Yellow Leaf Curl Virus Disease Ty-1 to Tomato[J].Northern Horticulture,2012,36(23):136.
[2]孙国珍,高利利,陆文利,等.河北省设施番茄褪绿病毒分子检测和鉴定研究[J].北方园艺,2015,39(09):95.[doi:10.11937/bfyy.201509026]
　SUN Guo-zhen,GAO Li-li,LU Wen-li,et al.Molecular Detection and Identification of Tomato chlorosis virus Infecting Greenhouse-grown Tomato Plants in Hebei Province[J].Northern Horticulture,2015,39(23):95.[doi:10.11937/bfyy.201509026]
[3]金凤媚,薛俊,宋建,等.番茄抗病基因Sw-5和Ty-3a的多重PCR检测体系的建立[J].北方园艺,2017,41(11):115.[doi:10.11937/bfyy.201711023]
　JIN Fengmei,XUE Jun,SONG Jian,et al.Identification of Resistance Gene Sw-5 and Ty-3a by Multiplex PCR in Tomato[J].Northern Horticulture,2017,41(23):115.[doi:10.11937/bfyy.201711023]
[4]谢文娟,吴钰坡,黄江,等.罗汉果性别相关的SCAR标记筛选[J].北方园艺,2019,43(08):32.[doi:10.11937/bfyy.20183623]
　XIE Wenjuan,WU Yupo,HUANG Jiang,et al.Screening of SCAR Markers Linked to Sex Determination in Siraitia grosvenorii (Swingle) C.Jeffrey ex Lu et Z.Y.Zhang[J].Northern Horticulture,2019,43(23):32.[doi:10.11937/bfyy.20183623]

备注/Memo

第一作者简介：董盼盼（1989-），女，山东惠民人，硕士研究生，研究方向为蔬菜生化与分子生物学。
责任作者：王富（1966-），男，黑龙江明水人，博士，教授，研究方向为蔬菜遗传育种及生物技术。E-mail:wangfuabcd@163.com.
基金项目：山东省自然科学基金资助项目（ZR2010CM047）；山东省现代农业产业技术体系资助项目；山东省良种工程资助项目（621294）；青岛市民生计划资助项目（13-1-3-3-nsh）。
收稿日期：2013-09-13

更新日期/Last Update: 2014-08-26