XIE Wenjuan,WU Yupo,HUANG Jiang,et al.Screening of SCAR Markers Linked to Sex Determination in Siraitia grosvenorii (Swingle) C.Jeffrey ex Lu et Z.Y.Zhang[J].Northern Horticulture,2019,43(08):32-39.[doi:10.11937/bfyy.20183623]
罗汉果性别相关的SCAR标记筛选
- Title:
- Screening of SCAR Markers Linked to Sex Determination in Siraitia grosvenorii (Swingle) C.Jeffrey ex Lu et Z.Y.Zhang
- Keywords:
- Siraitia grosvenorii; sex determination; molecular markers; RAPD; SCAR
- 文献标志码:
- A
- 摘要:
- 以罗汉果雌雄单株各30份为试材，采用随机引物筛选法，筛选与罗汉果性别相关的RAPD标记，并根据特异片段的测序结果，将RAPD标记转化为特异性和稳定性更好的SCAR标记，以期为罗汉果的性别早期鉴定和分子辅助育种研究提供参考依据。结果表明：在330条随机引物中获得了分别与罗汉果雌性和雄性相关的RAPD引物S2124和S343。根据特异序列测序结果，对雌性特异带S2124-1K和雄性特异带S343-1.4K各自设计并合成了2对SCAR引物，经验证，SCAR引物SC2124-1K-14在62 ℃和SC2124-1K-8在56 ℃均可扩增出1 019 bp的雌性特异带，SC343-1.4K-12在64 ℃和SC343-1.4K-4在56 ℃均可扩增出1 373 bp的雄性特异带。这些特异的SCAR标记具有较好的应用价值，可为罗汉果的性别早期鉴定提供分子依据。
- Abstract:
- Thirty female and male individual plants of S.grosvenorii respectively were used as experimental materials.RAPD markers related to sex determination of S.grosvenorii by random primers were screened,and transformed them into SCAR markers based on the sequencing results of specific bands.The results showed that two markers obtained from 330 RAPD primers,S2124 and S343,which related to female and male of S.grosvenorii respectively.According to the sequencing results of specific fragments,four pairs of SCAR markers were synthesized.By testing,the SCAR markers SC2124-1K-14 and SC2124-1K-8 could amplify 1 019 bp female-specific fragment on 62 ℃ and 56 ℃ respectively,SC343-1.4K-12 and SC343-1.4K-4 could amplify 1 373 bp male-specific fragment on 64 ℃ and 56 ℃ respectively.These SCAR markers had promotional values,and they could provide molecular foundation for gender identification of S.grosvenorii in seedling stage.
参考文献/References:
[1]中华人民共和国卫生部药典委员会.中华人民共和国药典[M].北京:化学工业出版社,2005:147.[2]周延清.DNA分子标记技术在植物研究中的应用[M].北京:化学工业出版社,2005:85-86.[3]黄龙花,吴清平,杨小兵,等.基于特定引物PCR的DNA分子标记技术研究进展[J].生物技术通报,2011(2):61-65.[4]魏丽娟,张娟,刘林德,等.南蛇藤雌性性别相关的RAPD和SCAR标记[J].植物研究,2014,34(5):687-693.[5]戴正,陈力耕,童品璋.香榧性别鉴定的RAPD和SCAR标记[J].果树学报,2008,25(6):856-859.[6]李仕金,辛培尧,郭鸿彦,等.大麻雄性相关RAPD和SCAR标记的研究[J].广东农业科学,2012(24):151-154.[7]景建洲,金红,李东亮,等.谭清苏铁性别连锁的RAPD和SCAR分子标记[J].生物工程学报,2007,23(6):1097-1101.[8]任朝兴,黄建昌,肖艳,等.番木瓜雄性性别的RAPD和SCAR标记[J].果树学报,2007,24(1):72-75.[9]YANG L Q,FU S,KHAN M A,et al.Molecular cloning and development of RAPD-SCAR markers for Dimocarpus longan variety authentication[J].Springer Plus,2013(2):501.[10]WU Y J,ZHANG Z M,CHEN Y,et al.Authentication of Thailand jasmine rice using RAPD and SCAR methods[J].Eur Food Res Technol,2009,229:515-521.[11]THYE S C,KABEN A M,TSE S C.Identification and characterization of RAPD-SCAR markers linked to glyphosate-susceptible and -resistant biotypes of Eleusine indica (L.) Gaertn[J].Mol Biol Rep,2014,41:823-831.[12]KALIA P,SAHA P,RAY S.Development of RAPD and ISSR derived SCAR markers linked to Xca1Bo gene conferring resistance to black rot disease in cauliflower (Brassica oleracea var.botrytis L.)[J].Euphytica,2017,213:232.[13]KIM D K,SEO S G,KWON S B,et al.Development of RAPD and SCAR markers related to watermelon mosaic virus and zucchini yellow mosaic virus resistance in Cucurbita moschata[J].Hortic Environ Biotechnol,2016,57(1):61-68.[14]王剑锴,李明杰,王建明,等.金线莲RAPD-SCAR标记的开发和种质遗传多样性评价[J].中草药,2016,1(47):122-129.[15]周涛,谢宇,张丽艳,等.基于SCAR分子标记的头花蓼分子鉴定研究[J].中国中药杂志,2013,38(16):2577-2580.[16]孙保娟,李植良,黎振兴,等.SCAR标记转化失败的原因和对策[J].分子植物育种,2010,8(3):589-594.[17]VIDAL J R,DELAVAULT P,COARER M,et al.Design of grapevine (Vitis vinifera L.) cultivar-specific SCAR primer for PCR fingerprinting[J].Theor Appl Genet,2000,101(8):1194-1201.[18]李萌,贺竹梅.遗传学教学中性别决定关键基因的阐述[J].遗传,2014,36(6):611-617.[19]赵志刚,柳美玲.黄瓜性别决定的分子机理研究进展[J].武汉纺织大学学报,2012,25(6):65-69.[20]吴起顺,杨贵春,韩永忠,等.薄皮甜瓜性型分化的遗传分析[J].北方园艺,2013(19):10-15.[21]陈瑶瑶,严良文,刘智成,等.苦瓜性别分化研究进展[J].福建农业科技,2014(9):69-72.[22]宁志怨,董玲,李卫文,等.栝楼性别的分化与鉴定研究进展[J].江苏农业科学,2014,42(8):4-6.
相似文献/References:
[1]马克群,徐函兵,范宏伟.夏季遮荫对罗汉果生长和净光合速率的影响[J].北方园艺,2014,38(09):35.
MA Ke-qun,XU Han-bing,FAN Hong-wei.Effect of Shading on Growth and Net Photosynthetic Rate of Siraitia grosvenorii in Summer[J].Northern Horticulture,2014,38(08):35.
[2]李旭,曹万万,姜丹,等.基于形态观察法和生理生化法的软枣猕猴桃[J].北方园艺,2014,38(24):6.
LI Xu,CAO Wan-wan,JIANG Dan,et al.Study on Sex Identification of Actinidia arguta by Morphology and Physiological Biochemical[J].Northern Horticulture,2014,38(08):6.
[3]陈 继 富,田 启 健.罗汉果组培苗在湖南湘西地区的引种研究[J].北方园艺,2010,34(21):0.[doi:10.11937/bfyy.201021071]
CHEN Ji -fu,TIAN Qi-jian.Study on Introduction of the Siraitia grosvenorii Seedling in Xiangxi Area of Hunan Province[J].Northern Horticulture,2010,34(08):0.[doi:10.11937/bfyy.201021071]
[4]蓝群,金晨钟,莫亿伟.罗汉果块根粗提取物抗氧化及抑菌能力[J].北方园艺,2018,42(10):144.[doi:10.11937/bfyy.20173116]
LAN Qun,JIN Chenzhong,MO Yiwei.Antioxidant and Antimicrobial Activities of Crude Extractions Components From Tuberous Root of Siraitia grosvenorii(swingle)[J].Northern Horticulture,2018,42(08):144.[doi:10.11937/bfyy.20173116]
[5]韦荣昌,覃芳,唐美琼,等.茉莉酸甲酯对罗汉果SQS、CS和CAS基因表达的影响[J].北方园艺,2019,43(02):42.[doi:10.11937/bfyy.20182039]
WEI Rongchang,QIN Fang,TANG Meiqiong,et al.Effect of Methyl Jasmonate on Expression of SQS,CS and CAS Genes in Siraitia grosvenorii[J].Northern Horticulture,2019,43(08):42.[doi:10.11937/bfyy.20182039]
备注/Memo
第一作者简介：谢文娟（1974-），女，博士研究生，研究方向为药用植物资源开发与利用。E-mail：xwj90029188@163.com.责任作者：黄江（1977-），男，博士，副教授，研究方向为植物分子生物学。E-mail：hjiang1997@126.com.基金项目：广西高校科学技术研究重点资助项目（ZD2014097）；广西自然科学基金面上资助项目（2013GXNSFAA019084）；国家自然科学基金资助项目（81560619）。收稿日期：2018-12-03