XIAN Li-jie,LIU Xing-ju,LI Xue-yan,et al.SSR Analysis of Genetic Diversity and Fingerprint Database Construction of Grape Germplasm[J].Northern Horticulture,2013,37(11):87-90.
葡萄种质遗传多样性的SSR分析及指纹库构建
- Title:
- SSR Analysis of Genetic Diversity and Fingerprint Database Construction of Grape Germplasm
- 文章编号:
- 1001-0009(2013)11-0087-04
- Keywords:
- grape; SSR; genetic diversity; fingerprint database
- 分类号:
- S 663.1
- 文献标志码:
- A
- 摘要:
- 以取自郑州果树研究所的45个葡萄品种为试材，利用SSR标记技术对其进行了遗传多样性分析。结果表明：利用19对基本核心引物对位于不同染色体上的SSR位点进行PCR扩增，共扩增出76条带，其中包括74条多态性条带。每对引物可检测到等位位点数为2~5个，多态率为66.7%~100%。通过聚类分析结果表明，在遗传距离为0.41处，45份葡萄材料可分为三大类群。对供试葡萄品种的不同引物的扩增产物进行检测，得到了每个品种在一组位于不同染色体的SSR位点上的基因型图谱，从而初步建立一个供试葡萄品种的SSR基因型指纹库。这为利用SSR标记鉴定葡萄品种或品系提供了基础数据。
- Abstract:
- Taking 45 grape varieties collected from Zhengzhou Fruit Research Institute as materials,the genetic analysis of them were analyzed using SSR markers technology.The results showed that 19 basic core primers for PCR amplification of SSR loci were located on different chromosomes,76 bands were amplified,including 74 polymorphic bands.No.of allelic loci detected by each pair of primer ranged from 2 to 5,with the polymorphic rate of 66.7%~100%.Through the cluster analysis showed that 45 grape materials could be divided into 3 groups in the genetic distance of 0.41.Detection of different primers amplified products of the grape varieties tested,the genotype maps of each species in a set of SSR loci in different chromosomes were gotten.Grape varieties SSR genotype fingerprint library were preliminarily established,which provided a basic data for identification of grape varieties or lines using SSR markers.
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备注/Memo
第一作者简介：宪立杰（1987-），女，硕士研究生，现主要从事树木分子标记及葡萄指纹图库的建立等研究工作。
责任作者：梁海永（1973-），男，本科，副教授，硕士生导师，现主要从事林业生物技术等研究工作。基金项目：国家林业局林业公益性行业科研专项资助项目（201104039）。
收稿日期：2013-01-16