WANG Bing-lin,HAN Tai-li,LI Yuan-yuan.Effects of Meloidogyne incognita Infection on Physiology and Biochemistry of Eggplant Seedling Leaves[J].Northern Horticulture,2012,36(23):139-142.
根结线虫侵染对茄子苗期生理生化反应的影响
- Title:
- Effects of Meloidogyne incognita Infection on Physiology and Biochemistry of Eggplant Seedling Leaves
- 文章编号:
- 1001-0009（2012）23-0139-04
- Keywords:
- eggplant; Meloidogyne incognita; seedling growth; SOD; POD; CAT; malondialdehyde(MDA)
- 分类号:
- S 641.1
- 文献标志码:
- A
- 摘要:
- 以“紫阳长茄”为试材，研究了根结线虫侵染对茄子苗期生长和叶片保护酶活性及膜脂过氧化的影响。结果表明：随着根结线虫侵染加剧，茄子幼苗生长受到严重抑制，根系和茎叶鲜重、干重及根系活力显著下降，株高、茎粗也有所降低，同时，茄子叶片保护酶活性和膜脂过氧化程度也发生了显著变化。超氧化物歧化酶（SOD）、过氧化物酶（POD）和过氧化氢酶（CAT）活性总体上均呈先升后降的趋势，但变化细节存在差异。SOD、CAT活性均在接种后10 d迅速上升，前者在接种后15 d达到峰值，之后下降，而后者则在接种后20 d达到峰值，随后下降；POD活性在接种后15 d急剧上升，在接种后20 d达到峰值，而后下降。根结线虫侵染还导致茄子叶片丙二醛（MDA）含量持续增加，在接种后15 d急剧上升，使细胞膜遭受伤害，促使植株早衰。
- Abstract:
- ‘Ziyangchangqie’ eggplant was used to study the effects of Meloidogyne incognita infection on seedlings growth，activities of protective enzymes and superoxidation of membrane lipid in leaves.The results indicated that the seedling growth was severely inhibited，and the root activity，and the fresh and dry weight of root，stem and leaf declined significantly with Meloidogyne incognita infection.Moreover，the plant height and stem diameter also decreased.On the whole，the activities of protective enzymes，namely，superoxide dismutase (SOD)，peroxidase (POD) and catalase (CAT)，increased at first but declined whereafter.The activities of SOD and CAT increased rapidly at 10 d post-inoculation (dpi)，and the activity of the former reached peak at 15 dpi，but declined whereafter，however，that of fhe later reached peak at 20 dpi.The activity of POD increased sharply at 15 dpi，and reached peak at 20 dpi，decreased subsequently.Furthermore，the Meloidogyne incognita infection also led to a persistent increase of the content of malondialdehyde (MDA) in leaves，and rised sharply at 15 dpi，which caused the cell membrane hurt and plant senescence early.
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备注/Memo
第一作者简介：王冰林（1972-），男，博士，副研究员，现主要从事蔬菜遗传育种和栽培生理研究工作。E-mail:binglinwang@126.com.