WANG Jin-lu,QIAN Tai-lin,ZHAO Mei-ai.Study on Cryopreservation of Lilium tsingtauense GilgLiliaCeae by Encapsulation-Vitrification Method[J].Northern Horticulture,2012,36(18):135-139.
青岛百合包埋-玻璃化法超低温保存技术研究
- Title:
- Study on Cryopreservation of Lilium tsingtauense GilgLiliaCeae by Encapsulation-Vitrification Method
- Keywords:
- Lilium tsingtauense GilgLiliaCeae; shoot tips; encapsulation-vitrification; cryopreservation
- 分类号:
- S 682.2+9
- 文献标志码:
- A1001-0009（2012）18-0135-05
- 摘要:
- 以青岛百合茎尖为试材，研究了外植体的选择、预培养的蔗糖浓度、预培养时间、包埋预处理方法、PVS2处理时间对百合存活率的影响。结果表明：小鳞茎在4℃冷锻炼培养28 d后，剥取2~3 mm的茎尖为试验材料，在蔗糖浓度0.4 mol/L的MS+100 g/L海藻糖+1 mg/L ABA的培养基上，预培养3 d，然后加0.4 mol/L蔗糖和2 mol/L甘油、3%褐藻酸钠进行包埋预处理，在0℃条件下用PVS2处理60 min，投入液氮保存1 h，38℃解冻2 min、洗涤后转接至恢复培养基上暗培养7 d，用TTC检测存活率最高可达到67.5%。
- Abstract:
- Taking shoot tips(2~3 mm) of Liliumtsingtauense GilgLiliaCeae.from 28 days cold-hardened bulblets at 4℃ as materials，effects of different explants，sucrose concentration of pre-culture，duration of pre-culture，different encapsulation pretreatment，duration of treatment with PVS2 were studied.The results showed that the most appropriate sucrose concentration was 0.4 mol/L during pre-incubation and the best pre-culture time was 3 d.Shoot-tips were encapsulated in 3% sodium alginate including 0.4 mol/L sucrose and 2 mol/L glycerol after pre-incubation，and PVS2 for 60 min at 0℃，plunged directly into liquid nitrogen for 1 h，then thawed for 2 min at 38℃and washed.At last，the shoot tips were cultured on recovery medium.The highest survival rate was 67.5 % with TTC staining method.
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备注/Memo
第一作者简介：王金录（1984-），男，山东德州人，在读硕士，研究方向为植物生化与分子生物学。E-mail：us293118@163.com.