LI Hong,CHANG Jian,ZHAO Jian,et al.Study on Volatile Oils of Lilium longiflorum by Different Extraction Methods with GC-MS[J].Northern Horticulture,2012,36(17):90-94.
铁炮百合花挥发油不同提取方法的GC-MS研究
- Title:
- Study on Volatile Oils of Lilium longiflorum by Different Extraction Methods with GC-MS
- 文章编号:
- 1001-0009（2012）17-0090-05
- Keywords:
- Lilium longiflorum; volatile oils; extraction methods; gas chromatography mass spectrometry(GC-MS)
- 分类号:
- Q 946
- 文献标志码:
- A
- 摘要:
- 采用气液相连续萃取法和水蒸气蒸馏法对铁炮百合花的挥发油进行提取，气相色谱-质谱法分离并鉴定了其化学成分；并用峰面积归一化法测定了各成分的百分含量。结果表明：2种方法共分离出57个组分，确定了其中52种化合物；其主要成分为烯、醇、酯、醛、酸和烷类化合物。与水蒸气蒸馏法相比，气液相连续萃取法多萃取出14种化合物，增加的化合物中低沸点、相对分子质量小的成分较多。另外，2种方法共有的低沸点化合物中，气液相连续萃取法萃取出的化合物含量均较高，如α-萜品醇（0.40%、3.49%）、（E）-3，7-二甲基-2，6-辛二烯-1-醇（0.35%、2.60%）、3，7，11-三甲基-1，6，10-十二碳三烯-3-醇（10.40%、19.76%）等。
- Abstract:
- The volatile oils was extracted by steam distillation method and gas liquid phase continual extraction method from Lilium longiflorum and by gas chromatography mass spectrometry(GC-MS) were analyzed.Finally the area normalization was used to measure the relative percentages of each of the components.The results showed that 57 components were separated and 52 components were identified.The main components were alkene,alcohol,ester,aldehyde,acid and alkane series.compared with steam distillation method,more than 14 components with lower boiling points and small relative molecular mass were extracted by gas liquid phase continual extraction method.The relative percentages were higher of lower boiling points components by gas liquid phase continual extraction method,for example：p-menth-1-en-8-ol(0.40%,3.49%),(E)-3,7-dimethyl-2,6-Octadien-1-ol(0.35%,2.60%),3,7,11-trimethyl-1,6,10-Dodecatrien-3-ol（10.40%,19.76%）.
参考文献/References:
[1]龙雅宜,张金政,张兰年.百合—球根花卉之王[M].北京:金盾出版社,1999:7-57. [2]郭朝晖,蒋生祥.中药百合的研究和应用[J].中医药学报,2004,32(3):27-29. [3]杨林莎,孙艳红,方晓艳.中药百合的研究进展[J].河南中医药学刊,2002,17(1):74-75. [4]赵秀玲.百合的营养成分与保健作用[J].中国野生植物资源,2010,29(1):44-46. [5]张继,马君义,姚健.西伯利亚百合花挥发油化学成分的研究[J].西北植物学报,2003(12):2184-2187. [6]张继,赵小亮,马君义.巴巴拉百合花的天然香气成分研究[J].西北植物学报,2005,25(4):786-790. [7]回瑞华,侯冬岩,李铁纯.GC/MS法分析百合花化学成分[J].鞍山师范学院学报,2003,5(2):61-63. [8]刘灿明,黄高山,刘祥华.超临界CO2萃取百合花挥发油的工艺研究[J].天然产物研究与开发,2005,17(4):485-487. [9]中华人民共和国卫生部药典委员会.中华人民共和国药典一部[M].北京:化学工业出版社,2010:附录XD. [10]施钧慧,汪聪慧.香料质谱图集[M].北京:中国质谱学会,1992. [11]北京工商大学.香料化学与工艺学[M].北京:化工工业出版社,2004:65-370.
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备注/Memo
第一作者简介：李红（1978-），女，硕士，助理研究员，现主要从事植物中挥发性成分研究工作。E-mail：lh_9214@163.com.