HU Li-zhi,YUAN Chun-long,YUAN Lin.The Method of Tannin-protein Precipitate to Determinate Content of Tannins in Grape Seed[J].Northern Horticulture,2012,36(14):23-26.
蛋白-单宁沉淀法测定葡萄籽中单宁含量
- Title:
- The Method of Tannin-protein Precipitate to Determinate Content of Tannins in Grape Seed
- 文章编号:
- 1001-0009(2012)14-0023-04
- Keywords:
- tannins; tannin-protein precipitate; precision; recovery rate; MDL
- 分类号:
- TS 261.7
- 文献标志码:
- A
- 摘要:
- 采用单宁-蛋白沉淀法测定葡萄籽超微粉中的单宁含量，分别从机器精密度，线性范围，方法检出限，方法精密度，加标回收率等方面进行比较，以研究葡萄籽超微粉中单宁含量的测定方法。结果表明：机器精密度1.144％,线性范围50~250 μg/mL(R2=0.9966),方法检出限0.339 μg/mL,平行性精密度0.2454％，重复性精密度0.2159％,加标回收率为97.47％~101.49％。该方法可用于葡萄籽超微粉中单宁含量的测定。
- Abstract:
- Using the method of tannin-protein precipitate to detect the content of tannins，the experimental condition from the precision of the machine，linearity，method detection limit (MDL)，accuracy and recovery to carry on the comparison were analyzed，in order to determinate the concentration of tannins in the ultrafine powder of grape seed.The results showed that the linearity of correlation of catechin were good in the range of 50~250 μg/mL (R2=0.9966)，the precision of the machine was 1.144％，MDL 0.339 μg/mL，the parallel precision 0.2454％，the repeatability precision 0.2159％ and the recovery rate was 97.47％~101.49％.The method was able to determine the content of tannins in the ultrafine powder of grape seed.
参考文献/References:
[1]李莹,苏婷婷,王战勇.葡萄加工副产品的综合利用研究[J].中国农学通报,2006,22(4):106-108.
[2]李银平,薛雪萍,袁春龙,等.葡萄籽成分与营养评价[J].食品与发酵工业,2006,32(12):108-113.
[3]李华,袁春龙,沈洁.超微粉碎技术在葡萄籽加工中的应用[J].华南理工大学学报,2007,35(4):123-126.
[4]薛雪萍,李华,袁春龙,等.葡萄籽超微粉的结块性研究[J].西北农林科技大学学报,2007,35(6)：104-107.
[5]熊何健,周常义,郑新阳,等.葡萄籽多酚对高脂膳食小鼠降血脂和抗氧化功能的影响[J].江西农业学报,2008,20(1):105-107.
[6]陆兴熠,刘剑英,钟进义.葡多酚对核辐射接触人员氧化损伤防护作用[J].中国公共卫生,2008,24(9):53-54.
[7]舒啸尘,李悠慧,严卫星,等.葡萄籽多酚在拟衰老模型小鼠中抗氧化功能的研究[J].卫生研究,2002,31(3):4550,54.
[8]丰佃娟,徐贵发.葡萄籽提取物对人体抗氧化能力的影响[J].山东大学学报,2007,45(10):18-20.
[9]Harold J，Blytt，Timothy K，et al.Antinutritional effects and ecological significance of dietary condensed tannins may not be due to binding and Xinhibiting digestive enzymes[J].Journal of chemical ecology,1988,6(14):1455-1465.
[10]王书园,俞凌云,朱云.单宁的分析研究现状[J].江苏调味副食品,2008,25(5):16-19.
[11]Harbertson J.Kennedy F J A，Adams D O.Change of tannins in grape skin and seed of Cabernet Sauvignon，Syrah and Pinot Noir during ripening period[J].Am J Enol Vitic,2002,53:54-59.
[12]陈桂芳,倪建秀,周国勤.日本沼虾中磺胺类药物残留量测定的检出限问题[J].江苏农业科学,2009(4):346-349.
[13]GB/T 5750.3-2006,生活饮用水标准检验方法,水质分析质量控制[S]．
[14]李义,禹海青,董建芳,等.加标回收在水质分析中的应用及回收率计算方法[J].岩矿测试,2010,29(5):124127.
[15]American Public Health Association.Standard methods for the examination of water and wastewater[S].19th Edition 1995,1010C,1030E.
[16]朱家平,王亚平,刘建坤.不确定度连续传递模型及其在化学测量中的应用[J].地质通报,2009,28(10)：1481-1485.
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备注/Memo
第一作者简介：胡立志（1985-），男，硕士，研究方向为葡萄酒化学。E-mail：hulizhi86362028@126.com.
责任作者：袁春龙（1969-），男，博士，副教授，硕士生导师，研究方向为葡萄酒化学。
基金项目：陕西省农业攻关资助项目（2011K02-04）；西北农林科技大学校人才支持计划资助项目（2111020901）。
收稿日期：2012-03-30