YIN Shu-li,ZHANG Li-ping,ZHANG Gen-wei,et al.The Synergism of Different Biocontrol Agents to Cucumber Fusarium wilt[J].Northern Horticulture,2012,36(07):151-155.
生防菌防治黄瓜枯萎病的协同作用研究
- Title:
- The Synergism of Different Biocontrol Agents to Cucumber Fusarium wilt
- 文章编号:
- 1001-0009（2012）07-0151-05
- 分类号:
- S 436.421.1+3
- 文献标志码:
- A
- 摘要:
- 采用传统方法统计可培养微生物的数量，采用常规方法测定土壤酶活、统计黄瓜形态指标和发病株数，探讨不同生防菌株枯草芽孢杆菌D、放线菌317和木霉菌联合防治黄瓜枯萎病是否具有协同增效作用。结果表明：3株菌株联合优于单一菌株和2株菌株联合，其协同增效作用最佳。与对照相比，3株菌株联合处理根际土壤中细菌、放线菌、真菌数量的接种增率分别为50.57%、110.98%和13.48%，菌群结构开始由真菌型向细菌型过渡；土壤中性磷酸酶、蔗糖酶、过氧化氢酶和脲酶活性增长率分别为244.90%、7.17%、2.90%和31.01%；黄瓜株高、茎粗、叶片数和干物质重量的增长率分别为33.92%、35.48%、34.43%和79.13%；防治效果最佳达52%。与其它处理相比，3株菌株联合处理对根际土壤微生物数量、菌群结构、过氧化氢酶、黄瓜的形态指标和枯萎病的防治效果具有明显的协同增效作用，其在生产中具有较大的应用潜力。
- Abstract:
- The number of culturable microorganisms were measured by traditional method.Soil enzyme activity，plant phenotypic traits and incidence number were determined by routine analysis methods,to explore the synergism of biocontrol agents bacteria D，actinomycetes 317 and fungi compound to cucumber Fusarium wilt.The results showed that three agents compound was superior to single agent and two agents compound，its synergism was the best.Three agents compound was compared to control；number of bacteria，actinomycetes and fungi could raise 50.57%，110.98% and 13.48%；microflora was becoming fungi type to bacteria type；activity of phosphatase，invertase，catalase and urease could raise 244.90%，7.17%，2.90% and 31.01%；plant height，stem diameter，leaf number and dry matter weight could raise 33.92%，35.48%，34.43% and 79.13%；control effect could raise 52%.Compared to control，three agents compound had clear synergism in number microorganism，microflora，plant phenotypic traits and control effect.The synergism to phosphatase，invertase and urease was not clear，but to catalase was clear.Three agents compound would have great application potential in production.
参考文献/References:
[1]Qin Y,Jin X N,Park H D.Comparison of antioxidant activities in black soybean preparation fermented with various microorganisms [J].Agricultural Sciences in China,2010,9(7):1065-1071.
相似文献/References:
[1]龚 娜,肖 军,杨 镇,等.不同菌根真菌对蓝莓根际土壤微生物数量的影响[J].北方园艺,2013,37(17):175.
GONG Na,XIAO Jun,YANG Zhen,et al.Effects of Different Mycorrhizal Fungi on Soil Microbial Number in the Blueberry Rhizosphere[J].Northern Horticulture,2013,37(07):175.
[2]尹淑丽.,麻耀华.,张丽萍.,等.不同生防菌对黄瓜根际土壤微生物数量及土壤酶活性的影响[J].北方园艺,2012,36(01):10.
YIN Shu-li.,MA Yao-hua.,ZHANG Li-ping.,et al.Effects of Different Biocontrol Agents on Soil Microbial Number and Enzyme Activity in the Cucumber Rhizosphere[J].Northern Horticulture,2012,36(07):10.
[3]谢修鸿,刘玉伟,王晓红,等.孔雀草水提液对自身生长及土壤微生物数量和酶活性的影响[J].北方园艺,2015,39(06):157.[doi:10.11937/bfyy.201506044]
XIE Xiu-hong,LIU Yu-wei,WANG Xiao-hong,et al.Effect of Water Extraction Liquid of Tagetes patula L.on Itself Growth and Microbial Quantity and Enzymatic Activity[J].Northern Horticulture,2015,39(07):157.[doi:10.11937/bfyy.201506044]
[4]王胜永,李宝辉,赵林,等.生草对黄淮地区苹果园土壤理化性质和微生物的影响[J].北方园艺,2021,(04):87.[doi:10.11937/bfyy.20201831]
WANG Shengyong,LI Baohui,ZHAO Lin,et al.Effects of Grass on Soil Physical and Chemical Properties and Microorganism of Apple Orchard in Huanghuai Area[J].Northern Horticulture,2021,(07):87.[doi:10.11937/bfyy.20201831]
[5]曹旭,张先成,王向向,等.蚯蚓粪对番茄根际土壤微生物学特性的影响[J].北方园艺,2021,(15):97.[doi:10.11937/bfyy.20210280]
CAO Xu,ZHANG Xiancheng,WANG Xiangxiang,et al.Effects of Vermicompost on Microbial Characters in Rhizosphere Soil of Tomatoes[J].Northern Horticulture,2021,(07):97.[doi:10.11937/bfyy.20210280]
备注/Memo
第一作者简介：尹淑丽（1979-），女，河北沧州人，硕士，助理研究员，现主要从事农业生物防治等方面的研究工作。E-mail:yslbaihelr@126.com。