WANG Shu-bin,QUAN Xue-li,ZHOU Yu-hong,et al.Establishment of Plantlet Regeneration System of Cotyledon Node for Astragalus membranaceus[J].Northern Horticulture,2012,36(06):177-178.
膜荚黄芪子叶节植株再生体系的建立
- Title:
- Establishment of Plantlet Regeneration System of Cotyledon Node for Astragalus membranaceus
- 文章编号:
- 1001-0009(2012)06-0177-02
- Keywords:
- Astragalus membranaceus; cotyledon node; regeneration system
- 分类号:
- S 567.5+3
- 文献标志码:
- A
- 摘要:
- 以膜荚黄芪无菌苗子叶节为外植体，建立了简单、高效和稳定的膜荚黄芪再生体系。结果表明：不定芽诱导的最适培养基是MS+6-BA 1.0 mg/L+NAA 0.10 mg/L，每个子叶节外植体平均出芽数为3个以上，再生频率达75.5%；生根最适培养基为1/2MS+IBA 0.5 mg/L；移栽后成活率达75%以上。
- Abstract:
- The cotyledon node obtained from aseptic seedling of Astragalus membranaceus were used as the explant and a simple，stable and high efficient regeneration system for A.membranaceus were established.The results showed that the optimum medium for adventitious shoot regeneration was MS+6-BA 1.0 mg/L+NAA 0.10 mg/L，the average number of differential buds per cotyledon node was above 3，and the regenerated frequency was 75.5%.The suitable rooting medium was 1/2MS+IBA 0.5 mg/L and the survive rate was above 75% after transplantation.
参考文献/References:
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备注/Memo
第一作者简介：王树斌(1986-)，男，山东烟台人，硕士，现主要从事园艺植物生物技术研究工作。