WAN Shu-yan,BAO Xiu-feng.Study on Rapid Propagation Techniques of Dianthus caryophyllus[J].Northern Horticulture,2012,36(06):114-117.
香石竹快速繁殖技术研究
- Title:
- Study on Rapid Propagation Techniques of Dianthus caryophyllus
- 文章编号:
- 1001-0009（2012）06-0114-04
- Keywords:
- Dianthus caryophyllus; apical bud; internodes with axillary bud; non-internode stem sements
- 分类号:
- S 681.503.8
- 文献标志码:
- A
- 摘要:
- 以中花散枝香石竹“红芭芭拉”的茎段为外植体，探讨不同培养基对香石竹的诱芽萌动、继代增殖和生根培养的影响。结果表明：外植体切取部位的诱导芽萌动率从高至低排序为：顶芽＞带腋芽的茎段＞不带腋芽的茎段；当培养基为MS+6-BA 1.0 mg/L时，对香石竹带腋芽的茎段芽萌动最好；在培养基为1/2 MS+6-BA 1.0 mg/L+NAA 0.2 mg/L时，继代增殖长芽的效果最好；在培养基为1/2 MS+6-BA 0.2 mg/L+NAA 0.5 mg/L时，诱导不定根效果最好。
- Abstract:
- With the stem of Dianthus caryophyllus as explants,the effects of different media on the shoot bud induction，subculture and rooting were investigated. The results showed that the parts of the explants induced shoots sprout rate from high to low order was terminal bud>internode with axillary buds>without internodes of the stem; on the medium MS+6-BA 1.0 mg/L,the internode with axillary buds to sprout was the best; on the medium 1/2MS+6-BA 1.0 mg/L+NAA 0.2 mg/L, subculture sprouting was the best and on the medium 1/2MS+6-BA 0.2 mg/L+NAA 0.5 mg/L，the induction of adventitious roots was the best.
参考文献/References:
[1]徐颂军,李娘军.认识花草[M].广州:广东人民出版社,2002.
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备注/Memo
第一作者简介：宛淑艳（1968-），女，黑龙江肇东人，博士，副教授，现主要从事植物功能基因及植物组织培养工作。E-mail：yuanwen-2005@163.com。