GAN Zhong-xiang,LI Bei-jin,ZHANG Lu-xia,et al.Study on Immature Seed Culture in Processing Tomato[J].Northern Horticulture,2012,36(05):137-138.
加工番茄未成熟种子培养
- Title:
- Study on Immature Seed Culture in Processing Tomato
- 文章编号:
- 1001-0009（2012）05-0137-02
- Keywords:
- processing tomato; immature seeds; tissue culture
- 分类号:
- S 641.204+.1
- 文献标志码:
- A
- 摘要:
- 为了提高加工番茄育种效率，取加工番茄授粉后14~30 d的果实，剥离果胶取未成熟种子在不同培养基上诱导出芽，从而摸索培养条件和移栽方法。结果表明：在1/2B5或1/2MS培养基上诱导出芽，授粉后25 d左右的未成熟种子发芽率较高，从培养未成熟种子到移栽需20 d，移栽至营养钵到缓苗需15 d，再移栽至大田。一般大田从授粉到收获需要60 d左右，如组培每代可节省50~60 d，可实现一年3~5代。
- Abstract:
- In order to speed up processing tomato breeding，the immature seeds were collected 14~30 d after pollination in processing tomatoes.The immature seeds were grown on the different media under different culture conditions.The transplanting methods were also tested in this study.The results showed that immature seed germination rate was high on 1/2B5 or 1/2MS medium about 25 d after pollination.It took 20 days from the immature seeds culutre to transplanting.The seedlings can be strong after 15 days after transplanting,and then transplanted to field.Generally，it took 60 days from pollination to harvesting seeds.Therefore，each generation growth period by immature seeds culture could be saved 50~60 d.And 3~5 generations can be ontained in 1 year by this method.
参考文献/References:
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[2]张录霞,郝青南,马超,等.加工番茄遗传转化再生体系的建立[J].西北农业学报,2008,17(3):236-241.
[3]吴志刚,宋明,王志敏,等.番茄组织培养中无菌苗培养条件的优化[J].中国农学通报,2006(22):335-337.
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备注/Memo
第一作者简介：甘中祥（1978-），男，本科，农艺师，现从事加工番茄育种研究工作。
责任作者：彭刚(1964-)，男，本科，高级农艺师，现主要从事加工番茄育种研究工作。E-mail：pengyz@cofco.com。
基金项目：中粮屯河股份有限公司资助项目。
收稿日期：2011-11-28