LI Hui,FAN Jun-feng,GAO Jian-she,et al.Establishment of Tissue Culture Regeneration System of Populus tomentosa Clone-30[J].Northern Horticulture,2012,36(03):110-113.
毛白杨无性系30号组培再生体系的建立
- Title:
- Establishment of Tissue Culture Regeneration System of Populus tomentosa Clone-30
- 文章编号:
- 1001-0009（2012）03-0110-04
- Keywords:
- populous tomentosa colne-30; proliferation; rootage; tissue culture
- 分类号:
- S 792.117
- 文献标志码:
- A
- 摘要:
- 以毛白杨无性系30号的茎段为外植体，对其再生能力和再生条件进行了研究，以期建立毛白杨无性系30号的组织快繁体系。结果表明：MS培养基为比较合适的基本培养基；MS+6-BA 0.5 mg/L和MS+TDZ 0.0015 mg/L+NAA 0.01 mg/L是其比较合适的诱导培养基，不定芽诱导率分别为86.7%和83.3%；MS+6-BA 0.7 mg/L+NAA 0.05 mg/L是其最佳增殖培养基，增殖率为628%；诱导毛白杨30号生根的最佳培养基为1/2MS+NAA 0.01 mg/L，生根率为96.8%。
- Abstract:
- Taking stems of the P.tomentosa clone-30 as explants, the regeneration ability and conditions were studied,in order to build a rapid tissue propagation system.The results showed that the appropriate basic medium was MS and the appropriate inductive medium was MS+6-BA 0.5 mg/L and MS+TDZ 0.0015 mg/L+NAA 0.01 mg/L，the shoot induction rates were respectively 86.7% and 83.3%.The optimal regeneration medium was MS+6-BA 0.7 mg/L+NAA 0.05 mg/L and the regeneration rate was 628%,the optimal rootage medium was 1/2MS+NAA 0.01 mg/L，and the rate of the rooting was 96.8%。
参考文献/References:
[1]高建社.杨树新品种-14、30、85号毛白杨[J].现代种业,2003(4)：47.
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备注/Memo
第一作者简介：李慧(1985-)，女，在读硕士，现主要从事林木遗传育种和林业生物技术研究工作。 E-mail：lh200108@163.com。