ZHANG Yu-qin.Screening and Identification of Bacillus subtilus Strain Bs-18 and Its Biocontrol Evaluation Against Meloidogyne incognita[J].Northern Horticulture,2012,36(01):130-132.
枯草芽孢杆菌Bs-18菌株分离与鉴定及对根结线虫的防治效果
- Title:
- Screening and Identification of Bacillus subtilus Strain Bs-18 and Its Biocontrol Evaluation Against Meloidogyne incognita
- 文章编号:
- 1001-0009(2012)01-0130-03
- Keywords:
- Bacillus subtilus; Meloidogyne incognita; screening; identification
- 分类号:
- S 432.4+5
- 文献标志码:
- A
- 摘要:
- 从植物的根际土壤中分离到1株芽孢杆菌菌株Bs-18，通过16S rDNA序列分析鉴定为枯草芽孢杆菌(Bacillus subtilus)。在室内，通过浸泡法用Bs-18菌株发酵液处理南方根结线虫(Meloidogyne incognita) 的2龄幼虫，发现其对线虫具有显著的致死作用，发酵原液及10倍稀释液在6 h时致死率均高于95%。在温室试验中，Bs-18菌株发酵液及10倍稀释液对根结线虫的减退率达到81.8%和76.5%，表现出较高的防治效果，为进一步的田间应用奠定了基础。
- Abstract:
- Astrain Bs-18 was got from the soil surrounding plant root,and based on the blast of 16S rDNA sequence, the strain Bs-18 was identified as Bacillus subtilus.In vitro,the root-knot nematodes (Meloidogyne incognita) second stage juveniles (J2) were treated with bacterial suspension of strain Bs-18. Responding to 6 hours treatment, the motility of nematode reached to 99.8% and 95.2% in the bacterial suspension and 10 times dilution of strain Bs-18 respectively. In greenhouse, the quantity of gall and egg mass of the strain Bs-18 treatment were decreased significantly, the galls of 81.8% and 76.5% were less in the treatment of suspension and 10 times dilution of Bs-18 than in the control group. This revealed that the nematocide effectiveness the strain Bs-18 was obviously.
参考文献/References:
[1]Abad P,Gouzy J,Aury J M,et al.Genome sequence of the metazoan plant-parasitic nematode Meloidogyne incognita[J].Nat Biotechnol,2008,26:909-915.
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备注/Memo
作者简介：张玉芹（1974-），女，硕士，讲师，现从事园艺教学及研究工作。E-mail:zhangyuqin345@163.com。