[1]李荣华,郭培国,张华,等.高温胁迫对不同耐热性菜心材料生理特性的差异研究[J].北方园艺,2012,36(01):1-6.
　LI Rong-hua,GUO Pei-guo,ZHANG Hua,et al.Effects of Heat Stress on Several Physiological Traits in Heat-yolerant and Heat-Sensitive Genotypes of Flowering Chinese Cabbage[J].Northern Horticulture,2012,36(01):1-6.
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高温胁迫对不同耐热性菜心材料生理特性的差异研究

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第36卷 期数: 2012年01期 页码: 1-6 栏目: 出版日期: 2012-01-16
Title:
Effects of Heat Stress on Several Physiological Traits in Heat-yolerant and Heat-Sensitive Genotypes of Flowering Chinese Cabbage
文章编号:
1001-0009(2012)01-0001-06
作者:
李荣华1; 郭培国1; 张华2; 黄红弟2; 郑岩松2; 夏岩石1
1.广州大学 生命科学学院，广东 广州 510006；
2.广州市农业科学研究院，广东 广州 510308
Author(s):
LI Rong-hua1;  GUO Pei-guo1;  ZHANG Hua2;  HUANG Hong-di2;  ZHENG Yan-song2;  XIA Yan-shi1
1.College of Life Sciences,Guangzhou University,Guangzhou,Guangdong 510006;?
2.Guangzhou Academy of Agricultural Sciences,Guangzhou,Guangdong 510308
关键词:
菜心; 高温胁迫; 渗透调节物质; 活性氧; 清除
Keywords:
flowering Chinese cabbage (Brassica campestris L. ssp. chinensis var. utilis Tsen et Lee);  heat stress;  osmolyte;  active oxygen;  scavenging
分类号:
S 634.5
文献标志码:
A
摘要:
以4个耐热性存在差异的菜心品种（系）为试材，研究持续高温胁迫对菜心叶片中渗透调节物质、电解质渗漏率、活性氧的产生与清除的影响。结果表明：短期（1 d）高温胁迫下，除过氧化氢酶（CAT）酶活性在耐热性弱的菜心材料中急剧增加，且达到活力最高值外，其它性状如脯氨酸和甜菜碱含量、电解质渗漏率、丙二醛（MDA）含量、O-?2产生速率、H2O2含量、超氧化物歧化酶（SOD）和过氧化物酶（POD）酶活性出现小幅度增加，且这些性状的值在4个菜心材料间差异不大;随着高温胁迫处理时间的延长（3～7 d），生理指标在4个材料中呈现不同程度的增加，表现出耐热性强的材料的甜菜碱含量、SOD、POD和CAT酶活性均明显高于耐热性弱的材料，电解质渗漏率、MDA含量、O-?2产生速率、H2O2含量则明显低于耐热性弱的材料；而脯氨酸含量的高低则未表现出与菜心耐热性强弱的对应关系。
Abstract:
Four flowering chinese cabbage were selected as material, electrolytic leakage rate and the production and scavenging of active oxygen in the leaves of heat-tolerant and heat-sensitive genotypes of flowering Chinese cabbage under heat stress were studied. The results showed that most of traits including the contents of proline and betaine, electrolytic leakage rate, malondialdehyde (MDA) content, O-?2producing rate, H2O2 content, activities of superoxide dismutase (SOD) and peroxidase (POD) increased slightly and were not significant differences under the short (1 d) period of heat stress in four genotypes of flowering Chinese cabbage, except catalase (CAT) which its activity rapidly increased and reached the peak in two heat-sensitive genotypes under 1 d of heat stress. The values of these physiological traits continuously increased with different degree in four genotypes with stress time (3～7 d.), and showed that the betaine content, the activities of SOD, POD and CAT in heat-tolerant genotypes were significant higher than that in heat-sensitive genotypes, electrolytic leakage rate, MDA content, O-?2 producing rate and H2O2 content were significant lower than that in sensitive genotypes, while proline accumulation was not related to heat tolerance or heat sensitive in flowering Chinese cabbage under heat stress condition.

参考文献/References:

[1]张华, 刘自珠.菜薹（菜心）的市场需求与育种现状[J].中国蔬菜,2010(3):10-12

.[2]Lobell D B, Asner G P. Climate and management contributions to recent. trends in U.S. agricultural yields [J].Science,2003,299(5609):1032
[3]曹毅,李春梅,邓燏,等.不同菜心品种耐热性研究[J].西南师范大学学报(自然科学版),2010,35(5):128-131.
[4]何晓明,潘瑞炽,廖飞雄.菜心耐热变异体离体筛选研究[J].广东农业科学,1999(5):17-18.
[5]廖飞雄,潘瑞炽.热胁迫下菜心脯氨酸含量变化及其在耐热中的作用[J].华南师范大学学报(自然科学版),2001(2):45-48.
[6]廖飞雄,潘瑞炽.菜心耐羟脯氨酸变异筛选方法的研究[J].江西农业大学学报,2003,25(6):875-878.
[7]廖飞雄,潘瑞炽.菜心耐羟脯氨酸初选系的耐热性[J].热带亚热带植物学报,2004,12(4):359-362.
[8]廖飞雄,潘瑞炽.羟脯氨酸对离体培养的菜薹幼苗、茎尖和愈伤组织的生理效应[J].植物生理学通讯,2004,40(1):56.
[9]李植良,孙保娟,罗少波,等.高温胁迫下华南茄子的耐热性表现及其鉴定指标的筛选 [J].植物遗传资源学报,2009,10(2):244-248
[10]Mishra V,Srivastava G,Prasad S M.Antioxidant response of bitter gourd (Momordica charantia L.) seedlings to interactive effect of dimethoate and UV-B irradiation [J]. Scientia Horticulturae,2009,120:373-378.
[11]郝再彬,苍晶,徐仲.植物生理实验技术[M].哈尔滨:哈尔滨工业大学出版社,2004:101-116.
[12]王爱国,罗广华.植物的超氧物自由基与羟胺反应的定量关系[J].植物生理学通讯,1990,39(6):55-57.
[13]郭培国,李荣华.夜间高温胁迫下对水稻叶片光合机构的影响[J].植物学报,2000,47(7):13-18.
[14]Bradford M.A rapid and sensitive method for the quantitation of microgram. quantities of protein utilizing the principle of protein-dye binding[J]. Anal Biochem,1976,72:248-254.
[15]Giannopolitis C N, Ries S K. Superoxide dismutase .I. Occurrence in higher plants[J]. Plant Physiol,1977,59:309-314.
[16]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000:25-28.
[17]郭培国,宋波龙,许兰桂,等.光谱法测定植物组织中甜菜碱含量方法的改良[J].广州大学学报（自然科学版）,2011,10(3):32-36.
[18]Martineau J R, Specht J E, Williams J H,et al. Temperature tolerance. in soybeans. I. Evaluation of a technique for assessing cellular membrane thermostability [J].Crop Science,1979,19:75-78.
[19]Guo P,Baum M,Grando S,et al.Differentially expressed genes between. drought-tolerant and drought-sensitive barley genotypes in response to drought stress during the reproductive stage[J].Journal of Experimental Botany,2009,60(12):3531-3544.
[20]Wahid A, Gelani S, Ashraf M,et al.Heat Tolerance in plants: An overview.[J].Environmental and Experimental Botany,2007,61:199-223.
[21]Essemine J,Ammar S, Bouzid S. Impact of heat stress on germination and growth in higher plants: physiological, biochemical and molecular repercussions and mechanisms of defence [J]. Journal of Biological Sciences,2010,10(6):565-572.
[22]李敏,王维华,王然,等.高温胁迫对菠菜叶片保护酶活性和膜透性的影响[J].园艺学报,2004,31(1):99-100.
[23]贾志银,巩振辉,许红娟,等.高温胁迫对辣椒幼苗生长及生理性状的影响[J].北方园艺,2010(12):5-8.[24]Savchenko G E, Klyuchareva E A, Abrabchik L M, et al.Effect of periodic heat shock on the membrane system of etioplasts[J]. Russ J Plant Physiol,2002,49:349-359.
[25]Fokar M,Nguyen H T, Blum A.Heat tolerance in spring wheat. I. Estimating cellular thermotolerance and heritability[J].Euphytica,1998,104:1-8.
[26]Hasan M A,Ahmed J U, Bahadur M M, et al.Effect of late planting heat stress on membrane thermostability, proline content and heat susceptibility index of different wheat cultivars[J].J. NatnScLFoundation Sri Lanka., 2007,35(2):109-117.
[27]Wahid A, Close T J.Expression of dehydrins under heat stress and their relationship with water relations of sugarcane leaves[J].Biol Plant,2007,51:104-109.
[28]耶兴元,马锋旺,王顺才,等.高温胁迫对猕猴桃幼苗叶片某些生理效应的影响[J].西北农业科技大学学报(自然科学版),2004,32(12):33-37.

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备注/Memo

第一作者简介：李荣华（1966-），女，硕士，实验师，现从事作物逆境生物学和分子育种的研究工作。E-mail: ronghua@gzhu.edu.cn。

责任作者：郭培国（1963-），男，博士，教授，现从事作物逆境生物学和分子育种的教学和研究工作。E-mail: guopg@gzhu.edu.cn；guopg@yahoo.com。
基金项目：国家自然科学基金资助项目（30871526）；广州市科技支撑计划资助项目（2009Z1-E801）。
收稿日期：2011-09-29

更新日期/Last Update: 2014-08-22