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Molecular Identification and Antimicrobial Activity of Endophytic Fungi From Cyclocarya paliurus (Batal.) Iljinsk

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2022年10
Page:
95-101
Research Field:
Publishing date:

Info

Title:
Molecular Identification and Antimicrobial Activity of Endophytic Fungi From Cyclocarya paliurus (Batal.) Iljinsk
Author(s):
PAN Ke1; TAN Xiaoming1; 2; ZHAO Lichun1; 2; ZHONG Yuanjian1; YANG Yuting1
(1.College of Pharmacy,Guangxi University of Chinese Medicine,Nanning,Guangxi 530200；2.Guangxi Engineering Technology Research Center of Advantage Chinese Patent Drug and Ethnic Drug Development,Nanning,Guangxi 530200)
Keywords:
Cyclocarya paliurus(Batal.) Iljinsk; endophytic fungi; molecular identification; diversity analysis; antibacterial activity
PACS:
-
DOI:
10.11937/bfyy.20214376
Abstract:
The wild Cyclocarya paliurus (Batal.) Iljinsk in Baise，Guangxi was used as the test material,the genetic diversity and antibacterial activity of endophytic fungi from C.paliurus，in Baise Guangxi，were studied by means of fungal morphology，rDNA-ITS and antibacterial test，in order to understand the different population structure of endophytic fungi and screen out antibacterial activity of strains.The results showed that 76 strains，belonging to 25 genera，19 families，12 orders，5 classes，and 2 phyla were isolated from various tissues of C.paliurus，of which Fusarium、Pestalotiopsis and Diaporthe were the dominant genera.The diversity of endophytic fungi was significantly different among roots，stems，phloem and xylem，the Shannon index (H′=1.605 6) and root (1.016 7) of endophytic fungi in stem were much higher than in phloem (0.209 7) and xylem (0.266 7).The results of antibacterial activity showed that five strains of endophytic fungi inhibited at least one indicator pathogen.Among them，Pezicula sp.826 had the strongest inhibitory effect on Staphylococcus aureus and Escherichia coli，and Pezicula sp.927 had the strongest inhibitory effect on Candida tropicalis.According to the results of this study，there were abundant and diverse endophytic fungi and endophytic fungi with strong antibacterial activity in the precious medicinal plant C.paliurus，which could provide resources for the search of new antibacterial substances.

References:

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Last Update: 2022-07-04