|Table of Contents|

Effects of Humic Acid on Soil Nutrients and Microbial Community Structure of Greenhouse Cucumber Rhizosphere Under Salt Stress

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2022年05
Page:
83-90
Research Field:
Publishing date:

Info

Title:
Effects of Humic Acid on Soil Nutrients and Microbial Community Structure of Greenhouse Cucumber Rhizosphere Under Salt Stress
Author(s):
SUN Shijun1; MA Bo2; WANG Yixuan3; HAO Shuiyuan1
(1.Department of Agriculture,Hetao College，Bayannaoer，Inner Mongolia 015000；2.Bayannaoer City Cultivated Land Quality Monitoring and Protection Center，Bayannaoer，Inner Mongolia 015000;3.Bayannaoer City Academy of Agriculture and Animal Husbandry Science，Bayannaoer，Inner Mongolia 015000)
Keywords:
humic acid; cucumber; salt stress; microorganism
PACS:
-
DOI:
10.11937/bfyy.20213551
Abstract:
Taking ‘Jinyou No.35’ cucumber as the test material，pot experiment was used,the salt stress was simulated with NaCl concentration of 150 mmol?L-1.The effects of humic acid on soil nutrients and microbial community under salt stress were studied by adding humic acid with volume ratio of 300，600 times and 900 times,in order to explore the effects of humic acid on soil microecological environment of cucumber in greenhouse under salt stress.The results showed that humic acid significantly increased the contents of organic matter，available potassium，available phosphorus and total nitrogen in soil，but decreased the pH.In terms of bacterial community structure，there was no significant difference in bacterial α diversity among treatments.The relative abundances of Actinobacteria，Chloroflexi and Planctomycetes in T1 and T2 were higher than CK，while the relative abundances of Bacteroidetes and Acidobacteria were opposite.The changes of bacterial composition at the genus level was not obvious.In terms of fungal community structure，the OTU number and Shannon index of fungi in T1 and T2 were not significantly with CK，while the Chao1 index was significantly higher than CK.Humic acid also significantly increased the relative abundance of Ascomycota in T1，T2 and T3，which were 28.07%，26.48% and 6.41% higher than CK，and reduced the relative abundance of Basidiomycota and Chytridiomycota.Among the changes of known genera，the relative abundance of Chaetomium and Acremonium increased，and the relative abundance of Rhizophydium decreased.RDA redundancy analysis showed that available potassium was the key environmental factor affecting bacterial community structure，while organic matter，available phosphorus and pH were the key factors affecting fungal community structure.In conclusion，humic acid had a positive effect on improving salt-stressed soil by changing the physical and chemical properties of the soil and the soil microbial ecosystem.

References:

[1]王艳萍,李松龄,秦艳,等.不同年限日光温室土壤盐分及养分动态研究[J].中国土壤与肥料,2011(4):5-7.[2]ZAREMANSEH H,AKBARI N,EISVAND H R,et al.The effect of humic acid on soil physicochemical and biological properties under salinity stress conditions in pot culture of satureja khuzistanica jamzad[J].Ecopersia.2020,8(3):147-154.[3]高云晓,庞元湘,毛培利,等.黄腐酸有机肥对盐胁迫下刺槐幼苗生长的影响[J].西南林业大学学报(自然科学版),2019,39(2):36-43.[4]KHALED H，FAWY H A.Effect of different levels of humic acids on the nutrient content，plant growth，and soil properties under conditions of salinity[J].Soil and Water Research，2011(1)：21-29.[5]王德领,诸葛玉平,杨全刚,等.3种改良剂对滨海盐碱地土壤理化性状及玉米生长的影响[J].农业资源与环境学报,2021,38(1):20-27.[6]LI Y，FANG F，WEI J,et al.Humic acid fertilizer improved soil properties and soil microbial diversity of continuous cropping peanut：A three-year experiment[J].Scientific Reports，2019，9(1):12-15.[7]裴瑞杰,王俊忠,冀建华,等.腐殖酸肥料与氮肥配施对土壤理化性质的影响[J].江苏农业科学,2018,46(19):331-334.[8]孔令冉,董雯昕,杨天一,等.腐植酸在改良和培肥土壤中的作用[J].腐植酸,2019(1):7-12.[9]刘宇锋,罗佳,苏天明,等.外源腐殖酸对栽培基质性状和辣椒生长发育的影响[J].江苏农业学报,2016,32(3):647-655.[10]韩剑宏,孙一博,张连科,等.生物炭与腐殖酸配施对盐碱土理化性质的影响[J].干旱地区农业研究,2020,38(6):121-127.[11]张慧,李文卿,方宇,等.施用不同肥料对烟草土壤细菌群落的影响[J].安徽农学通报,2019,25(10):29-35.[12]聂天宏,杨兴,李永春,等.高分子材料在土壤物理性质改良方面的研究进展[J].土壤通报,2020,51(6):1504-1512.[13]ZENG J，LIU X，SONG L，et al.Nitrogen fertilization directly affects soilbacterial diversity and indirectly affects bacterial community composition[J].Soil Biology & Biochemistry，2016,92:41-49.[14]赵立君,刘云根,王妍,等.典型高原湖滨带底泥细菌群落结构及多样性特征[J].微生物学通报,2020,47(2):401-410.[15]王颖,孙层层,周际海,等.生物炭添加对半干旱区土壤细菌群落的影响[J].中国环境科学,2019,39(5):2170-2179.[16]张慧敏,郭慧娟,侯振安.不同盐碱胁迫对土壤细菌群落结构的影响[J].新疆农业科学,2018,55(6):1074-1084.[17]林少颖,陈桂香,刘旭阳,等.互花米草入侵对河口湿地土壤细菌群落结构及多样性影响[J].环境科学学报,2020,40(8):3001-3012.[18]袁婉潼.生物腐殖酸对盐碱土壤微生物数量和酶活性的影响[J].安徽农业科学,2016,44(1):1-4,45.[19]叶协锋,凌爱芬,刘清华,等.腐殖酸对植烟土壤理化性状影响的研究[J].中国农业科技导报,2010,12(6):120-125.[20]李鹏,李永春,史加亮,等.水稻秸秆还田时间对土壤真菌群落结构的影响[J].生态学报,2017,37(13):4309-4317.[21]FREY S D，KNORR M，PARRENT J L，et al.Chronic nitrogen enrichment affects the structure and function of the soil microbial community in temperate hardwood and pine forests[J].Forest Ecology and Management，2004，196(1)：159-171.[22]LAUBER C L，STRICKLAND M S，BRADFORD M A，et al.The influence of soil properties on the structure of bacterial and fungal communities across land-use types[J]．Soil Biology and Biochemistry，2008，40(9)：2407-2415．[23]HODGE A，ROBINSON D，FITTER A.Are microorganisms more effective than plants at competing for nitrogen?〖KG-*4〗[J].Trends in Plant Science，2000，5(7)：304-308.[24]LI F H，DING Z T，KE W C，et al.Ferulic acid esterase-producinglactic acid bacteria and cellulase pretreatments of corn stalk silage at twodifferent temperatures：Ensiling characteristics，carbohydratescomposition and enzymatic saccharification[J].Bioresource Technology，2019,282：211-221.[25]IZUMIKAKA M，KHAN S T，KOMAKI H，et al.JBIR-37 and-38，novel glycosylbenzenediols,isolated from the sponge-derived fungus，Acremonium sp.SpF080624G1f01[J].Bioscience，Biotechnology，and Biochemistry，2009，73(9)：2138-2140.

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Last Update: 2022-05-24