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Optimization of Medium for the Liquid Strain Using Response Surface Methodology (RSM) of Morchella sextelata

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2022年02
Page:
111-120
Research Field:
Publishing date:

Info

Title:
Optimization of Medium for the Liquid Strain Using Response Surface Methodology (RSM) of Morchella sextelata
Author(s):
KANG Chao; ZHENG Xuan; YANG Ling; ZENG Weijun; WANG Wankun; XIANG Zhun
(Guizhou Institute of Biology,Guiyang,Guizhou 550009)
Keywords:
Morchella sextelata; experiment design; sodium carboxymethylcellulose; liquid spawn
PACS:
-
DOI:
10.11937/bfyy.20212962
Abstract:
aking M.sextelata strain GZCC0287 as the test material,carbon source,nitrogen source,inorganic salt and sodium carboxymethylcellulose (CMC-Na) were investigate by single factor design,Plackett-Burman design,and central composite design for biomass weight and concentration of mycelium pellet,respectively,in order to provide an effective way for produce the liquid spawns,solid strain and industrial fermentation.The results showed that brown sugar,yeast powder,KH2PO4,MgCl2·6H2O were optimal carbon source,nitrogen source and inorganic salt individually.And the optimized medium combination was brown sugar 50.49 g·L-1,yeast powder 10 g·L-1,KH2PO4 1 g·L-1,MgCl2·6H2O 2.37 g·L-1,CMC-Na 10.11 g·L-1.Under this condition,the maximum biomass weight was (5.21±0.45)g and the concentration of mycelia pellets was 2 567±127 per milliliter.The mycelium of Morchella spp.filled the cultivation bag for 10 days to 15 days by inoculated liquid seed 5 mL for 1 000 per milliliter.

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Last Update: 2022-04-08