|Table of Contents|

Induction of Autotetraploid for Wild Kiwifruit

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2020年15
Page:
42-47
Research Field:
Publishing date:

Info

Title:
Induction of Autotetraploid for Wild Kiwifruit
Author(s):
YAO Pengqiang; QU Xuelian; CHENG Shiping
(School of Chemical and Environment Engineering,Pingdingshan University,Pingdingshan,Henan 467000)
Keywords:
wild kiwifruit; leaves in vitro; somatic chromosome doubling; autotetraploid
PACS:
-
DOI:
10.11937/bfyy.20194173
Abstract:
Wild kiwifruit were used as materials which located in Funiu mountain,Pingdingshan city,Henan Province.The method of using colchicine to induce the somatic chromosome doubling in leaves in vitro was adopted.The effects of different preculture time and colchicine solution to the induction efficiency of autotetraploid were investigated.This research try to get the best induction condition of autotetraploid for kiwifruit.The results showed that the suitable induction culture medium of adventitious bud was MS+3.0 mg?L-1 6-BA+0.2 mg?L-1 NAA with 88.00% differentiation rate.The suitable culture medium of rooting was 1/2MS+1.0 mg?L-1 IBA with 86.67% rooting rate.The optimal treatment combination of tetraploid induction was 50 mg?L-1 colchicine solution with 72 hours culture after three days preculture leaves.The induction rate was about 18.52%.19 tetraploids were induced by this method.The ploidy level could remain stable after three subculture.

References:

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Last Update: 2020-10-28