|Table of Contents|

Identification and Expression Analysis of E3 Ubiquitin Ligase Gene From Flammulina filiformis

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2020年05
Page:
129-137
Research Field:
Publishing date:

Info

Title:
Identification and Expression Analysis of E3 Ubiquitin Ligase Gene From Flammulina filiformis
Author(s):
WU Xin1; 2; WEN Die1; 2; HUANG Qianhui2; XIE Baogui1; 2; WEN Zhiqiang1; 2
(1.College of Life Science,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002；2.Mycological Research Center,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002)
Keywords:
E3 ubiquitin ligase; real-time quantitative fluroescence PCR; expression; stipe elogation
PACS:
-
DOI:
10.11937/bfyy.20193050
Abstract:
Based on the data of genomic and transcriptome data from Flammulina velutipes,the Flammulina filiformis′s putative protein E3 ubiquitin ligase gene was identified,named 〖STBX〗GENE5116〖STBZ〗,and its structure was analyzed,basic properties and expression pattern of the encoded protein,for understanding their role in the growth and development of Flammulina velutipes fruit bodies.The results showed that the gene had 9 exons and 8 introns,with a total length of 1 998 bp,encoding a total of 438 amino acid residues,a relative molecular mass of 48 987.36,and an isoelectric point of 8.83.Phylogenetic analysis showed that the gene had a high degree of homology with the fungal E3 ubiquitin ligase and had a closest relationship with Cylindrobasidium torrendii.The results of real-time fluorescence quantitative PCR showed that the gene was most highly expressed in the stalk and the gene expression in the upper part of the stalk was significantly higher than that in the lower part,the gene had the lowest expression in the mycelium.Therefore,it was speculated that the gene might be related to the elongation of the stipe.

References:

[1]李杨,李栋.泛素连接酶:底物选择关系的研究进展[J].生物技术通报,2015,31(1):11-20.[2]陈科,程汉华,周荣家.自噬与泛素化蛋白降解途径的分子机制及其功能[J].遗传,2012,34(1):7-20.[3]常笑君,郭玉琼,王仲,等.铁观音茶树RING型E3泛素连接酶基因克隆及其干旱胁迫下的表达分析[J].福建农业学报,2016,31(8):826-832.[4]秘彩莉,刘旭,张学勇.F-box蛋白质在植物生长发育中的功能[J].遗传,2006(10):1337-1342.[5]CRAIG K L,TYERS M.The F-box:A new motif for ubiquitin dependent proteolysis in cell cycle regulation and signal transduction[J].Prog Biophys Mol Biol,1999,72(3):299-328.[6]CALLIS J,VIERSTRA R D.Protein degradation in signaling[J].Curr Opin Plant Biol,2000,3(5):381-386.[7]宋素胜,谢道昕.泛素蛋白酶体途径及其对植物生长发育的调控[J].植物学通报,2006,23(5):564-577.[8]高飞.E3泛素连接酶亚基MogrrA调控稻瘟病菌生长发育的分子机制[D].合肥:安徽农业大学,2014.[9]张蕾.泛素连接酶RNF13对小鼠行为的影响及其分子机制的初步探讨[D].北京:中国协和医科大学,2008.[10]YAMADA M,SAKURABA S,SHIBATA K,et al.Cloning and characterization of a gene coding for a hydrophobin,Fv-hyd1,specifically expressed during fruiting body development in the basidiomycete Flammulina velutipes[J].Applied Microbiology & Biotechnology,2005,67(2):240-246.[11]KIM H I,LEE C S,PARK Y J.Further characterization of hydrophobin genes in genome of Flammulina velutipes[J].Mycoscience,2016,57(5):320-325.[12]董晨.香蕉β-淀粉酶基因家族的系统进化分析[J].热带作物学报,2016,37(11):2176-2182.[13]LIVAK K,SCHMITTGEN T.Analysis of relative gene expression data using real-time quantitative PCR and the 2-△△Ct Method[J].Methods,2000,25(4):402-408.[14]MORTAZAVI A,WILLIAMS B A,MCCUE K,et al.Mapping and quantifying mammalian transcriptomes by RNA-Seq[J].Nature Methods,2008，5(7)：621-628.[15]张艳芳,孙瑞芬,郭树春,等.向日葵E3泛素连接酶基因克隆及表达分析[J].西北植物学报,2016,36(10):1933-194.

Memo

Memo:
-
Last Update: 2020-05-03