|Table of Contents|

Changes of Defense Enzymes in Tomato Leaves of Inbred Lines With Different Resistance After TSWV Infection

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2020年03
Page:
44-4
Research Field:
Publishing date:

Info

Title:
Changes of Defense Enzymes in Tomato Leaves of Inbred Lines With Different Resistance After TSWV Infection
Author(s):
MAO Lianzhen; TANG Di; ZHU Haishan; ZHAO Kai
(College of Landscape and Horticulture,Yunnan Agricultural University,Kunming,Yunnan 650201)
Keywords:
tomato; tomato spotted wilt virus; disease resistance mechanism; physiological indicators; defense enzymes
PACS:
-
DOI:
10.11937/bfyy.20191735
Abstract:
Three tomato inbred lines with different resistance to tomato spot wilt virus (TSWV) were selected,namely YNAU335 (immunity),96172I (susceptibility) and No.5 (susceptibility).The changes of defense enzyme activity after inoculation of TSWV were studied by UV spectrophotometry,and to reveal the physiological mechanism of YNAU335 resistance to TSWV.The results showed that the activities of SOD,POD,CHT,β-1,3-GA and PPO in YNAU335 inbred lines were all higher than those in the inbred lines 96172I and No.5,while the activities of phenylalanine ammonia-lyase were lower than those in the two inbred lines;after TSWV infection,the activities of SOD,POD,CHT and β-1,3-GA in three inbred lines were higher than those in the control,while the activities of PPO were lower than those in the control.The activities of PAL in YNAU335 were higher than those in the control,while those in two susceptible inbred lines were lower than those in the control.After TSWV infection,the activities of six enzymes in YNAU335 were higher than those in the other two susceptible inbred lines.

References:

[1]陈坤荣,许泽永,晏立英,等.番茄斑萎病毒属(Tospovirus)病毒研究进展[J].中国油料作物学报,2005,27(3):91-96.[2]LOPEZ C,ARAMBURU J,GALIPIENSO L,et al.Evolutionary analysis of tomato Sw-5 resistance-breaking isolates of tomato spotted wilt virus[J].Journal of General Virology,2011,92(Pt 1):210.[3]PEIR A,CAIZARES M C,RUBIO L,et al.The movement protein (NSm) of tomato spotted wilt virus is the avirulence determinant in the tomato Sw-5 gene-based resistance[J].Molecular Plant Pathology,2015,15(8):802-813.[4]曹金强,谢学文,柴阿丽,等.番茄斑萎病毒病在宁夏的发现与防治[J].中国蔬菜,2016(4):87-89.[5]SPASSOVA M,PRINST W,FOLKERTSMA R T,et al.The tomato gene Sw-5 is a member of the coiled coil,nucleotide binding,leucine-rich repeat class of plant resistance genes and confers resistance to TSWV in tobacco[J].Molecular Breeding,2001(7):151-161.[6]DOCKTER K G,O′NEILD S,PRICE D L,et al.Molecular mapping of the tomato spotted wilt virus resistance gene “Sw-7” in tomato[C].2009 ASHS Annual Conference，2009.[7]SHI A N,VIERLING R,GRAZZINI R,et al.Identification of molecular markers for Sw-5 gene of tomato spotted wilt virus resistance[J].American Journal of Biochemistry and Biotechnology,2011,1(1):8-16.[8]吴梅珍,吴定华.栽培番茄与野生秘鲁番茄杂交胚胎发育的细胞学研究[J].华南农业大学学报,1991(4):29-36.[9]赵娟.梨果实愈伤组织对致腐真菌侵染的生理响应机制研究[D].杨凌:西北农林科技大学,2012.[10]张树生,胡蕾,刘忠良,等.植物体内抗病相关酶与植物抗病性的关系[J].安徽农学通报,2006,12(13):48-49.[11]莫云容,张培欣,邓明华,等.番茄自交系YNAU335对番茄斑萎病毒抗性的鉴定[J].中国蔬菜,2016(5):13-17.[12]SHAEMAN M,PERSLEY D M.Field isolates oftomato spotted wilt virusovercoming resistance in capsicum in Australia[J].Australasian Plant Pathology,2006,35(2):123-128.[13]冷月强,侯喜林.不结球白菜接种霜霉病菌后防御酶活性的变化[J].江苏农业学报,2006,22(3):305-307.[14]赵永发.抗番茄斑萎病毒物质筛选及作用机制初步研究[D].长沙:湖南农业大学,2011.[15]耿莉娜,龙艳玲,徐宸,等.感染赤星病后不同烟草品种叶片防御酶的变化[J].西南大学学报(自然科学版),2018,40(10):25-30.[16]左豫虎,康振生,杨传平,等.β-1,3-葡聚糖酶和几丁质酶活性与大豆对疫霉根腐病抗性的关系[J].植物病理学报,2009,39(6):600-607.[17]MONTALBINI P,BUONAURIO R.Effect of tobacco mosaic virus infection of levels of soluble superoxide dismutase (SOD) in nicotiana tabacum and nicotiana glutinosa leaves[J].Plant Science,1986,47(2):135-143.[18]匡传富,罗宽.烟草品种对青枯病抗病性及抗性机制的研究[J].湖南农业大学学报（自然科学版）,2002,28(5):395-398.

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Last Update: 2020-02-26