|Table of Contents|

Direct Regeneration of Adventitious Buds From Leaves of Rosa laevigata Michx.

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2019年13
Page:
93-100
Research Field:
Publishing date:

Info

Title:
Direct Regeneration of Adventitious Buds From Leaves of Rosa laevigata Michx.
Author(s):
XIAN Hongkang1; ZHI Qiujuan2; LI Hui2; WANG Changquan1
(1.College of Horticulture，Nanjing Agricultural University，Nanjing，Jiangsu 210095；2.Management Department of Hongmei Garden，Changzhou，Jiangsu 213003)
Keywords:
Rosa laevigata Michx.; adventitious bud; organogenesis; direct regeneration
PACS:
-
DOI:
10.11937/bfyy.20183979
Abstract:
The scratched leaves of tissue culture seedlings of Rosa laevigata Michx.were used as explants,and the effects of different combinations of plant growth regulators，dark culture time，additives L-proline and different parts of leaves on the direct regeneration of adventitious buds were studied.The results showed that TDZ concentration was 1.5 mg?L-1 and NAA concentration was 0.000 5 mg?L-1，the direct incidence of adventitious buds was the highest，which was 9.5%.Different dark culture time had certain influence on the direct induction of adventitious buds.The direct incidence of adventitious buds was the highest when dark culture time was 10 days，which was 10.5%.The induction rate of adventitious buds did not increase or decrease after adding different concentrations of L-proline.In comparison of regeneration rate of different parts of leaves，adventitious buds could be directly induced only with petioles.In conclusion，the method of inducing adventitious buds from leaves to regenerate directly was as follows：1/2MS+TDA 1.5 mg?L-1+NAA 0.000 5 mg?L-1+CH 100 mg?L-1+AgNO3 10 mg?L-1+sucrose 30 g?L-1+agar 7.5 g?L-1 on the direct induction medium with petioles.After 10 days of dark culture，the induction rate of adventitious buds was the highest (10.5%).The adventitious buds in the proliferation medium MS+NAA 0.1 mg?L-1+6-BA 1.0 mg?L-1grew after 3 weeks，the proliferation coefficient could reach about 3.5.Then the cluster buds were cut into single buds and transferred to MS medium to sound seedlings for 3 weeks.Subsequently，the resulted 3-5 cm seedlings were then transfer to MS+NAA 0.1 mg?L-1 medium for rooting,andthe post-rooting ratereached 95% one month later.

References:

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Last Update: 2019-08-02