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Title:: Effect of Different Conditions on the Change of Content of Osmotic Regulating Substances in Reed

Author(s):: YUAN Yunxiang1; 2; HE Boling1; 2; (1.College of Agriculture and Commerce,Weinan Normal University,Weinan,Shaanxi 714099；2.Shaanxi Province Engineering Research Center of Coal Conversion Alcohol,Weinan,Shaanxi 714099)

Abstract:: The reed of Shaanxi Yellow River Wetland was used as experimental material,the contents of free proline,soluble sugar and soluble protein in reed root,root tiller and upper leaf were determined by acid ninhydrin method,anthrone colorimetry and coomassie brilliant blue method.The physiological charactriristics of different parts of wetland reed were observed and compared by several groups of single factor experiments,such as changing the extraction time,changing the extraction temperature and so on.The environment changes in the growing zone of reed were studied，could provide a theoretical reference for protection of Shaanxi Yellow River Wetland.The results showed that the content of free praline of reed root and upper leaf was the highest when condition was extracting temperature 100 ℃,extracting time 10 minutes.The content of free praline of root tillerwas extracting temperature 80 ℃,extracting time 10 minutes.The content of soluble sugar of the upper leaves was significantly higher than reed root and root tiller.The content of soluble sugar was the highest when condition was extracting temperature 80 ℃,extracting time 40 minutes.The content of soluble protein was the highest under room temperature.It decreased as the temperature increased.