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Fluorescence Observation of Pollen Tube Growth and Embryo Culture From? Distant Hybridization Between Asiatic and Oriental Lily Cultivars

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2018年10
Page:
88-96
Research Field:
Publishing date:

Info

Title:
Fluorescence Observation of Pollen Tube Growth and Embryo Culture From? Distant Hybridization Between Asiatic and Oriental Lily Cultivars
Author(s):
WANG Chong1; HE Weili2; ZHANG Wei2; LEI Jiajun2
(1.Gardening College，Liaoning Forestry Vocation-technical College，Shengyang,Liaoning 110101；2.College of Horticulture，Shenyang Agricultural University，Shenyang,Liaoning 110866)
Keywords:
lily; distant hybridization; pollen tube growth; fluorescence observation; embryo culture
PACS:
-
DOI:
10.11937/bfyy.20173318
Abstract:
Pollen germination and pollen tube growth from distant reciprocal crosses between three different ploidy Asiatic lily cultivars，‘Brunello’ (4x)，‘Dazzling’ (3x) and ‘Pollyanna’ (2x)，and two diploid Oriental lily cultivars，‘Siberia’ (2x) and ‘Sorbonne’ (2x)，were observed under the fluorescence microscope.And the effect of different treatment methods on embryo germination and seedling was carried out in order to achieve lily new cultivar.The results showed that other pollens of lily cultivars could germinate except for the pollens on the stigma of ‘Dazzling’.But some abnormal phenomena of pollen tube growth appeared within 72 hours after pollination in most cross combinations，such as swelling on the terminal，twining or callose deposition in the pollen tubes，and most pollen tubes stopped growing in the style and lead to the failure to reach ovary.Only a few pollen tubes in ‘Brunello’בSiberia’，‘Brunello’בSorbonne’,‘Sorbonne’בBrunello’ and ‘Sorbonne’בPollyanna’ could reach the base of the style and finally enter into the ovary within 120 hours after pollination.The pollen tubes in ‘Siberia’בBrunello’，‘Siberia’בPollyanna’，‘Pollyanna’בSorbonne’ and ‘Pollyanna’בSiberia’ still grew slowly，but finally could not arrive at ovary within 120 hours after pollination.The interspecific hybrid seedlings were obtained via embryo rescue.The embryos without seed coat and endosperm，and the embryo age of 40 days after pollination were optimum for culture in vitro.The optimum medium for embryo culture was MS medium supplemented with 0.01 mg?L-1 NAA and 0.1 mg?L-1 BA. Keywords:lily；distant hybridization；pollen tube growth；fluorescence observation；embryo culture

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Last Update: 2018-06-11