|Table of Contents|

Factors Influencing Agrobacterium-mediated Genetic Transformation of Cucumber(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2018年02
Page:
9-14
Research Field:
Publishing date:

Info

Title:
Factors Influencing Agrobacterium-mediated Genetic Transformation of Cucumber
Author(s):
CHANG Huaicheng; NI Lei; SUN Yongdong; LUO Weirong
College of Horticulture and Landscape Architecture,Henan Institute of Science and Technology,Xinxiang,Henan 453003
Keywords:
cucumber; genetic transformation; Agrobacterium tumefaciens; CsEXP10
PACS:
-
DOI:
10.11937/bfyy.20173043
Abstract:
‘Jinyou No 1 ’ cucumber cotyledonary node explants was used as experimental material,the effects of seedling type,acetosyringone concentration （AS) in pre-culture medium and co-culture medium,washing times with sterile water,cephalosporin （Cef) concentration in select-culture medium and rooting medium on genetic transformation system were researched in order to supply reference for genetic transformation of cucumber.The results showed that various optimum parameters such as seedling type A（closed cotyledon),with 100 μmol?L-1 of AS,washed three times with sterile water,select-culture medium with 300 μmol?L-1 Cef,and rooting medium with 400 μmol?L-1 Cef were obtained with cotyledonary node explants.

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Last Update: 2018-01-26