|Table of Contents|

Cloning and Subcellular Localization Analysis of GbERF1 Transcription Factor in Ginkgo biloba L.

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2018年03
Page:
92-100
Research Field:
Publishing date:

Info

Title:
Cloning and Subcellular Localization Analysis of GbERF1 Transcription Factor in Ginkgo biloba L.
Author(s):
LI Zehong1; YUAN Honghui2; CHENG Hua2; LI Linling2; FENG Ru1; CHENG Shuiyuan1
1.School of Biology and Pharmaceutical Engineering,Wuhan Polytechnic University,Wuhan,Hubei 430023；2.College of Life Sciences,Huanggang Normal University,Huanggang,Hubei 438400
Keywords:
Ginkgo biloba L.; ERF transcription factors; fluorescence quantitative analysis; subcellular localization
PACS:
-
DOI:
10.11937/bfyy.20172419
Abstract:
ERF transcription factors are important in plants,which are widely involved in plant response to biotic and abiotic stress.Three-year-old Ginkgo biloba L. tree was used as materials,the sequences of ERF was explored by using RT-PCR and RACE-PCR technology,in order to understand the molecular mechanism of medicinal component metabolism of Ginkgo biloba L.The results showed that GbERF1 contained a 1 314 bp ORF encoding 437 amino acid residues.NCBI-BLAST showed GbERF1 protein had two specific hits of ERF superfamily.GbERF1shared 97% similarity with sequences of Picea sitchensis.Phylogenetic tree analysis showed that GbERF1 was grouped into the same clade with sequences of Picea sitchensis and Lupinus angustifolius.The expression patterns of GbERF1 genes in Ginkgo biloba L.tissues and under stress treatments were analyzed by real-time quantitative PCR,the results showed that GbERF1 genes expressed highly in root and leaf.The expression level of GbERF1 under the treatment of ET and UV was significant higher than that of control group,and expression trend of them was upgrade at first and then descend.The expression trend of them under drought was upgrade at first and then descend,and the expression difference was not significant under CCC treatment.Following transient expression of GbERF1 in epidermal cells of leaf of tobacco,GbERF1 was found to be localized in the nucleus.

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Last Update: 2018-02-09