|Table of Contents|

Comparative Study on Antioxidant Activity of Extracellular Polysaccharide,Intracellular Polysaccharide and Sclerotial Polysaccharide of Poria cocos

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2018年02
Page:
172-177
Research Field:
Publishing date:

Info

Title:
Comparative Study on Antioxidant Activity of Extracellular Polysaccharide,Intracellular Polysaccharide and Sclerotial Polysaccharide of Poria cocos
Author(s):
CHEN Lin; LIAO Yan; HUANG Wei; ZHANG Shuhang; LU Yaobang; CUI Peiwu
College of Pharmacy,Hunan University of Chinese Medicine,Changsha,Hunan 410208
Keywords:
polysaccharide of Poria cocos; antioxidant activity; infrared spectroscopy
PACS:
-
DOI:
10.11937/bfyy.20171329
Abstract:
In order to explore the antioxidant activity of different pachymaran,phenanthroline Fe2+ method,DPPH? assay method and pyrogallic acid self-oxidizing method were employed for analyzing ?OH scavenging activity,DPPH? scavenging activity and super oxygen anion free radical scavenging activity of extracellular polysaccharide,intracellular polysaccharide and sclerotial polysaccharide of Poria cocos.Then Fourier transform infrared spectrometry was also used to identify the structural difference among the three selected pachymaran.The results indicated that all the three pachymaran could exhibit scavenging activity of ?OH,DPPH? and O?[TX-*4]2,but intracellular polysaccharide showed the best antioxidant activity with ?OH scavenging activity of 32 07%,DPPH?scavenging activity of 90 12%,and super oxygen anion free radical scavenging activity of 39 74%,respectively.Analysis of infra-red spectra suggested that all the tested samples belonged to polysaccharide,and a special absorption peak at 823 cm-1 was only found in intracellular polysaccharide,which might imply the existence of mannose glycosidic bond.These data verified antioxidant activity of pachymaran and advantage of intracellular polysaccharide of Poria cocos in research and development of food and drug with antioxidant activity,and also provided a necessary reference for deep exploitation of fungal polysaccharide.

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Last Update: 2018-01-26