|Table of Contents|

Establishment and Optimization of cDNA-SRAP Reaction System by Orthogonal Design for Two Species of Lepidium L.(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年24
Page:
99-103
Research Field:
Publishing date:

Info

Title:
Establishment and Optimization of cDNA-SRAP Reaction System by Orthogonal Design for Two Species of Lepidium L.
Author(s):
LI Jia; ZHANG Na; LAI Xiaohui; LI Qun
(College of Life Science and Technology，Xinjiang University，Urumqi，Xinjiang 830046)
Keywords:
Lepidium L.; cDNA-SRAP; single-factor experiment; orthogonal experimental design
PACS:
-
DOI:
10.11937/bfyy.201624025
Abstract:
A cDNA sequence-related amplified polymorphism (SRAP) reaction system of two species Lepidium L.were established and optimiazed by orthogonal experimental design and single factor analysis of different concentration of dNTPs，Mg2+，primer，Taq DNA polymerase and DNA template was conducted.The results showed that the optimal reaction system for the two species of Lepidium L.was followed as 0.275 mmol?L-1 dNTPs，0.5 U Taq DNA polymerase，1.0 μmol?L-1 primer，100 ng template cDNA in total 20 μL of reaction solution.Using the optimal system of cDNA-SRAP,clear stable and repeatable bands could be amplified from two species of Lepidium plants for analyzing gene differential expression.

References:

 

[1]任珺,陶玲.新疆北部短命植物的特征分析[J].草业科学,2005,22(5):19-24.

[2]孙稚颖,李法曾.中国独行菜族(十字花科)部分属种的分子系统学研究[J].西北植物学报,2007,27(8):1674-1678.

[3]孟君,李群,李冠.两种独行菜种子萌发生理特性研究[J].生物技术,2008,18(2):32-35.

[4]袁祯燕,多力坤·买买提玉素甫,黄培佑.早春短命植物独行菜天然种衣与水分的关系[J].种子,2006,25(9):1-3.

[5]赵琼珍,师秋菊,李群.独行菜CBF基因的克隆及其植物表达载体的构建[J].种子,2012,31(4):4-6.

[6]葛凤伟,田永芝,曾卫军,等.独行菜eEF-1 a基因片段分离克隆及RT-PCR分析[J].新疆师范大学学报(自然科学版),2014(2):22-26.

[7]周茜,赵惠新,李萍萍,等.独行菜种子转录组的高通量测序及分析[J].中国生物工程杂志,2016,36(1):38-46.

[8]LI G,QUIROS C F.Sequence-related amplified polymorphism (SRAP)，a new marker system based on a simple PCR reaction:its application to mapping and gene tagging in Brassica[J].Theoretical and Applied Genetics,2001,103:455-461.

[9]吴建明,李杨瑞,杨柳,等.cDNA-AFLP和cDNA-SRAP技术在植物基因差异表达上的应用及其分析比较[J].生物技术通报,2009(11):52-55.

[10]QUE Y X,XU L P,LIN J W,et al.cDNA-SRAP and its application in differential gene expression analysis:a case study in Erianthus arundinaceum[J].Biomed Research International，2012,2012(1):390107.

[11]HUANG N,ZHANG Y Y,XIAO X H,et al.Identification of smut-responsive genes in sugarcane using cDNA-SRAP[J].Genetics and Molecular Research Gmr,2015,14(2):6808-6818.

[12]LIU C,YUAN D,ZHANG X,et al.Isolation，characterization and mappingof genes differentially expressed during fibre development between Gossypium hirsutum and G.barbadense by cDNA-SRAP[J].Journal of Genetics,2013,92(92):175-181.

[13]苏亮,白建荣,王秀红.玉米cDNA-SRAP反应体系的优化[J].山西农业科学,2010,38(8):6-9.

[14]何庆元,吴萍,张晓红,等.不同秋眠性苜蓿SRAP体系优化及遗传多样性分析[J].草业学报,2011,20(2):201-209.

[15]张四普,汪良驹,吕中伟.石榴叶片SRAP体系优化及其在白花芽变鉴定中的应用[J].西北植物学报,2010,30(5):911-917.

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Last Update: 2017-03-08