|Table of Contents|

Effect of NaCl Stress on Growth and Physiological Response of Limonium sinense in vitro(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年18
Page:
154-159
Research Field:
Publishing date:

Info

Title:
Effect of NaCl Stress on Growth and Physiological Response of Limonium sinense in vitro
Author(s):
LU Xingxia; GUO Wentao; SUN Yun; LAN Xiaotian; MING Cong; REN Zhiyu; LUO Jianxia; CHAI Cijiang; SUN Shihai
（College of Horticulture and Landscape，Tianjin Agricultural College，Tianjin 300384）
Keywords:
Limonium sinense (Girard) Kuntze; plantlet in vitro; salt stress; growth; physiological response
PACS:
-
DOI:
10.11937/bfyy.201618038
Abstract:
Differentiation seedlings was cultured on the MS proliferation and rooting medium with different NaCl concentration (0,02%,04%,06%,08%,10%)，and the growth (plant height，the number of leaves，leaf width，fresh weight，root length) and physiological (MDA,SOD,Pro,Na+，K+) indexes were measured and analyzed.The results showed that the growth of plantlet in vitro grew well under salt stress.With the NaCl stress increasing，the root length increased and was the longest in the NaCl concentration of 10%，and it was 39 times of the control；the plant height increased first then decreased and then increased again，it was the lowest in the NaCl concentration of 06% and the highest in the NaCl concentration of 08%，when the NaCl concentration was 10%，the plant height decreased to some extent，but the difference of the plant height was not significant between the NaCl concentration of 08% and 10%；the differenc of fresh weight in NaCl concentration of 02% was not significant compared with the control，the remaining treatments (NaCl concentration of 04%-10%) were significantly lower than the control，and the difference among them were not significant；the number of leaves decreased with the increase of the NaCl concentration，the number of leaves was the least in NaCl concentration of 10%，and was controlled by 1/3；the leaf width increased then decreased，the leaf width was the most narrow in the NaCl concentration of 04%，and the leaf width was the widest in the NaCl concentration of 08%，the difference of the leaf width was not significant between the NaCl concentration of 08% and 10%.The results of the physiological indexes showed that,MDA content，the activity of SOD and Pro content increased then decreased with the NaCl stress increasing.The contents of MDA were the highest and the lowest respectively in the NaCl concentration of 06% and 10%，and compared with the control，the contents of MDA were significantly higher in the NaCl concentration of 06% and lower significantly in the NaCl concentration of 10%；the contents of Pro were significantly higher than that of CK whether the NaCl concentration of 06% or 10%，and the contents of Pro was the highest in the NaCl concentration of 06%，and the contents of Pro decreased in the NaCl concentration of 10%；the activities of SOD were significantly higher than that of CK whether the NaCl concentration of 08% or 10%，and the activities of SOD was the highest in the NaCl concentration of 08%，and the second was the NaCl concentration of 10%.The contents of Na+ and K+ were all significantly higher that of CK，and also significantly increased with the NaCl stress increasing.With the NaCl stress increasing，ratio of K+/Na+ decreased then increased，ratio of K+/Na+ was the lowest in the NaCl concentration of 08%，and significantly increased in the NaCl concentration of 10%.In conclusion，L.sinense could endure as high as 10% NaCl，it could protect cell membrane system and enhance water absorption capacity to alleviating plant salinity injury by increasing the activities of SOD，the content of Pro and maintaining high K+/Na+ ratio.

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Last Update: 2016-11-01