|Table of Contents|

Determination of Three Phytosterols in Yam by Capillary Electrophoresis With  Electrochemical Detection(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年12
Page:
162-165
Research Field:
Publishing date:

Info

Title:
Determination of Three Phytosterols in Yam by Capillary Electrophoresis With  Electrochemical Detection
Author(s):
HU Yuefang
(College of Chemistry and Bioengineering,Hezhou University,Hezhou,Guangxi 542899)
Keywords:
capillary electrophoresis-electrochemical detection; yam; ergosterol; cholesterol; β-sitosterol
PACS:
-
DOI:
10.11937/bfyy.201612040
Abstract:
Taking yam as test material,a method of capillary electrophoresis coupled with electrochemical detection(CE-ED)was adopted for the determination of three phytosterol content (ergosterol,cholesterol,β-sitosterol)in yam.The effects of detection potential,concentration and pH of running buffer,separation voltage and injection time on the detection were investigated.The results showed that under the optimum conditions,a good baseline separation and highly sensitive detection were achieved in 10 minutes good linear relationship between peak currents and ergosterol ,cholesterol and β-sitosterol concentration ranging from 0.1-1 500 μg?L-1,0.1-1 000 μg?L-1,0.1-1 200 μg?L-1,with the detection limits(S/N=3)of 0.03 μg?L-1,0.04 μg?L-1,0.03 μg?L-1,respectively,the relative standard deviations(RSD)of peak currents were 1.5%,1.7%,1.6%,RSD of the migration time were 0.6%,0.7%,0.6%.The method was successfully applied to the assay of cholesterol,ergosterol and β-sitosterol in samples of yam.The spiked recoveries were in the range of 97.6%-100.3%,with relative standard deviations less than 2.3%．

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Last Update: 2016-07-21