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Effect of Different Hormone Combinations on the Induction,Proliferation and  Differentiation of Callus in Bletilla striata(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年12
Page:
157-161
Research Field:
Publishing date:

Info

Title:
Effect of Different Hormone Combinations on the Induction,Proliferation and  Differentiation of Callus in Bletilla striata
Author(s):
XU Delin1; SHEN Fang2; QIAN Gang1; QIAO Xiaoying2; CHU Shirun2; LI Lin1
(1.Department of Cell Biology,Zunyi Medical University,Zunyi,Guizhou 563000;2.The First Clinical Institute，Zunyi Medical University,Zunyi,Guizhou 563000)
Keywords:
Bletilla striata; callus; induction; proliferation; differentiation; medium
PACS:
-
DOI:
10.11937/bfyy.201612039
Abstract:
Bletilla striata(Thunb.)Rchb.f.was used in this study to screen the optimal mediums in callus induction,proliferation and differentiation through the comparisons of different hormones combined with the concentrations based on the medium of MS and 1/2MS.The results showed that the highest inducing rate was observed as 89.89% at the combination of MS+6-BA 1 mg?L-1+2,4-D 3 mg?L-1.6-BA(6-Benzylaminopurine)alone in the basic medium exhibited disability in producing the callus,while this disability was determined by adding 2,4-D(2,4-D ichlorophenoxyacetic acid)simultaneously.And the inducing rate increased as more 2,4-D was added,while the products quality decreased as the increasing of 2,4-D in the basic medium.Additionally,the combination of MS+6-BA 0.1 mg?L-1+2,4-D 2 mg?L-1 exhibited the highest proliferation rate(745.7%)than the other combinations after 30 days incubation.Increasing callus differentiation rate was observed as the increasing of NAA(alpha-Naphthylacetic acid)and TDZ(thidiazuron)in the basic medium,while NAA alone possessed better effect than TDZ.And the combination of MS+2,4-D 2 mg?L-1+NAA 1 mg?L-1 exhibited the highest differentiation rate(86.67%)than other combinations after 30 days differentiation.

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Last Update: 2016-07-21