|Table of Contents|

Tissue Culture of Endangered Plant Syringa pinnatifolia(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年12
Page:
92-96
Research Field:
Publishing date:

Info

Title:
Tissue Culture of Endangered Plant Syringa pinnatifolia
Author(s):
CHENG Ming1; LI Houhua2; HE Zisen1; JIANG Zaimin3; CAI Jing1
(1.College of Forestry,Northwest Agriculture and Forestry University,Yangling,Shaanxi 712100；2.College of Landscape Architecture and Arts,Northwest Agriculture and Forestry University,Yangling,Shaanxi 712100；3.College of Life Science,Northwest Agriculture and Forestry University,Yangling,Shaanxi 712100)
Keywords:
Syringa pinnatifolia; tissue culture; bud; seed
PACS:
-
DOI:
10.11937/bfyy.201612023
Abstract:
The buds and seeds of endangered plant Syringa pinnatifolia were used as the test materials,the influence of different disinfection methods,concentration combinations of sucrose and hormone on primary culture of seeds,combinations of basal media and 〖JP5〗hormones on shoots proliferation and rooting by plant tissue culture method were studied,in order to provide basis for tissue culture propagation,germplasm resources protection and exploitation of Syringa pinnatifolia.The results showed that the suitable explants were shoots.The appropriate disinfection methods were 75% ethanol for 30 seconds,0.1% mercuric chloride for 7 minutes,the best proliferation medium was MS+5.0 mg?L-1 6-BA+0.01 mg?L-1 IBA;the optimal initial,proliferation and rooting medium for seed was MS+20 g?L-1 sucrose+3.0 mg?L-1 6-BA+0.10 mg?L-1 IBA,MS+7.0 mg?L-1 6-BA+0.05 mg?L-1 IBA and WPM+2.0 mg?L-1 IBA respectively according to this study.

References:

 

[1]中国科学院《中国植物志》编辑委员会.中国植物志第61卷2分册[M].北京:科学出版社,1992:79.

[2]REFOUVELET E,LE N S,TALLON C,et al.A new method for in vitro propagation of lilac(Syringa vulgaris L.):regrowth and storage conditions for axillary buds encapsulated in alginate beads,development of a pre-acclimatisation stage[J].Scientia Horticulturae,1998,74:233-241.

[3]杨晓红,逯文亭,冷平生,等.不同种源华北紫丁香组培研究[J].北京农学院学报,2015,30(3):67-72.

[4]温瑀.裂叶朝鲜丁香组织培养体系的建立[D].哈尔滨:东北林业大学,2006.

[5]胡凯,张立军,白雪梅,等.植物组织培养污染原因分析及外植体的消毒[J].安徽农业科学,2007,35(3):680-681.

[6]姜在民,蔡靖,崔宏安.华榛、羽叶丁香种子形态构造特点的研究[J].陕西林业科技,1999(3):14-16.

[7]刘华英,沈海龙,黄剑.离体条件下暴马丁香切开种子的萌发[J].植物研究,2004,24(2):245-247.

[8]周莉,罗凤霞,代力民.丁香种间杂交幼胚离体培养研究[J].应用生态学报,2003,14(3):382-386.

[9]孟昕,陈春玲,代兴华.白丁香胚离体培养及快繁的研究[J].北京园林,2013,17(4):25-28.

[10]孟昕,陈进勇,赵世伟.佛手丁香胚培养的研究[C].中国植物园(第14期),2011.

[11]段乃彬,王永清.枣树胚离体培养的研究[J].四川林业科技,2002,23(2):42-45.

[12]韩丽媛,管少花,张娅君,等.酸枣成熟胚离体培养研究[J].河南农业科学,2013,42(10):118-121.

[13]刘建斌,赵祥云,王俊娟,等.紫丁香的组织培养[J].北京农学院学报,2001,16(4):42-45.

[14]武术杰.小叶丁香的再生体系建立[J].东北林业大学学报,2009,37(8):17-18.

[15]冷强,张竹.小叶丁香的组织培养[J].中国林副特产,2012(1):32-34.

[16]刘忠荣,陈屏昭.植物组织培养技术在观赏植物中的应用[J].昭通师范高等专科学校学报,2003,25(2):41-43,46.

[17]CORINA C,ADRIANA F,CORINA S,et al.In vitro propagation in Syringa josickaea Jacq.[J].Notulae Botanicae Horti Agrobotanici Cluj-Napoca,1998,1(28):71-77.

[18]谌金芳,何晓冰,张利,等.观赏树木组培愈伤组织分化研究进展[J].福建林业科技,2014,41(4):241-246.

Memo

Memo:
-
Last Update: 2016-07-21