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Screening，Identification and Antifungal Activity of BBC-3 Against Botrytis cinerea(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年06
Page:
84-88
Research Field:
Publishing date:

Info

Title:
Screening，Identification and Antifungal Activity of BBC-3 Against Botrytis cinerea
Author(s):
QIU Yanxiao1; 2; CHENG Huicai1; ZHOU Jing1; 2; YIN Shuli1; ZHANG Genwei1; ZHANG Liping1
(1.Institute of Biology，Hebei Academy of Sciences，Shijiazhuang，Hebei 050081；2.Shijiazhuang No.4 Pharmaceutical Co.Ltd.，Shijiazhuang，Hebei 052165)
Keywords:
Botrytis cinerea; Brevibacillus brevis; screen; identification; antifungal activity
PACS:
-
DOI:
10.11937/bfyy.201606021
Abstract:
aking cucumber rhizosphere soil as isolation objects，using dilution-plate method，BBC-3 was screened which had strong and stable antagonistic activity against Botrytis cinerea.The antifungal activity was identified by using confrontation culture and agar diffusion methods.The results showed that it was identified as Brevibacillus brevis based on the results of morphologic characteristics，physiological and biochemical properties and phylogenetic analysis of 16S rDNA sequence and was named for BBC-3.It had better activity to 14 kinds of plant pathogens.Antifungal substance from BBC-3 had high stability against heat，acid，alkali and irradiation of glare，and it was unsensible to proteinase.The antibacterial activity was more than 80% at 4℃ for 6 months.

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Last Update: 2016-05-03