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Regeneration System Establishment from Cotyledons in vitro of Cucumis sativus L.(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2014年19期
Page:
113-117
Research Field:
Publishing date:

Info

Title:
Regeneration System Establishment from Cotyledons in vitro of Cucumis sativus L.
Author(s):
LIU Yu-xia1; YE De-you2; OU Qiao-ming3; LI Min-quan 1; 4
1.Grassland College,Gansu Agricultural University,Lanzhou,Gansu 730070；
2.Institute of Vegetable,Gansu Academy of Agricultural Sciences,Scientific Observations Experiment Station of Biology and Genetic Improvement of Horticultural Crops,Ministry of Agriculture in the Northwest,Lanzhou,Gansu 730070;
3.Institute of Biotechnology Research,Gansu Academy of Agricultural Sciences,Lanzhou,Gansu 730070；4.Gansu Academy of Agricultural Sciences,Lanzhou,Gansu 730070
Keywords:
cucumber; in vitro; cotyledon; regeneration
PACS:
S 642.2
DOI:
-
Abstract:
Using three different ecotype cucumber varieties as experiment materials,the effect of explant types,combination of hormones in the medium and genotypes on cucumber plantlet regeneration were studied.The results showed that the ability of differentiation buds was cotyledon>hypocotyl>root.In the medium of M10（MS+1.5 mg/L 6-BA+2.0 mg/L AgNO3),three different ecotype cucumber varieties were shown a higher regeneration rate of adventitious buds.Among of the three varieties,european greenhouse cucumber “Excellent” had the highest shoot regeneration frequency which reached 14.71% and 2.0 shoots per explant cultured in M10 medium.S2（MS+0.1 mg/L 6-BA）medium could significantly promote the elongation of adventitious buds,MS medium supplemented with 0.15 mg/L IBA also could greatly make the regeneration of plant roots.

References:

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Last Update: 2014-11-25