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Mutagenesis of Mycelial of Cordyceps militaris and Selection of Fine Strains(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2013年08期
Page:
154-156
Research Field:
Publishing date:

Info

Title:
Mutagenesis of Mycelial of Cordyceps militaris and Selection of Fine Strains
Author(s):
ZHOU Xiao-dong; LI Ming; TIAN Jing-hua; LI Shou-mian; SUN Ya-jie; LIU Jun-bo
College of Horticulture，Agricultural University of Hebei，Baoding，Hebei 071001
Keywords:
Cordyceps militaris; mutagenesis; antagonism; esterase isozyme; yield; cordycepin
PACS:
S 567.3+5
DOI:
-
Abstract:
Taking Cordyceps militaris‘CM106’as material，the fragments from the mycelia of C.militaris ‘CM016’ were diluted to 104 times by using physiological saline cultured in liquid medium.Under a 20 W UV lamp，the fragments were mutated for 100 seconds with a distance of 32 cm.The effect of mutagenesis on selection of fine strains were studied.The results showed that 31 C.militaris strains whose mycelia had better morphological characteristics were obtained.Somatic incompatibility analysis indicated that 15 mutagenic C.militaris strains of all had antagonistic lines with the original strain.Further isozyme analysis showed that 11 of the 15 strains above had obvious differences with the original strain in genetic relationships.Of the 11 mutagenic strains above，8 were able to produce normal stromas.The results of the weight of production and content of cordycepin according to the statistics were that C.militaris C16 had a significantly higher production than the original strain，and C.militaris C6 had a significantly higher content of cordycepin than the original strain，respectively.

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Last Update: 2014-08-25