Study on Changes in Protein Components of Tonoplast from the Roots of Tomato Seedlings During Phosphate Starvation(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:

2013年07期

Page:

113-115

Research Field:

Publishing date:

Info

Title:

Study on Changes in Protein Components of Tonoplast from the Roots of Tomato Seedlings During Phosphate Starvation

Author(s):

XU Han-bing¹; 2; ZHU Qing-song²; SHAN Shu-hua¹; 3; LIU Jing-ru¹; SONG Ke-min¹

1.College of Plant Science and Technology，Huazhong Agricultural University，Wuhan，Hubei 430070；

2.Xinyang Agricultural College，Xinyang，Henan 464000；

3.Taiyuan Normal University，Taiyuan，Shanxi 030031

Keywords:

tomato; phosphate starvation; soluble proteins; tonoplast proteins

PACS:

Q 946.1

DOI:

-

Abstract:

The changes in tonoplast proteins and soluble proteins under Phosphate (Pi) starvation were studied in tomato seedlings growing by solution culture method.The results indicated that with the extension of Pi starvation time，the soluble protein contents increased significantly in tomato roots，but the changes in shoots were not remarkably.In addition，it was also noted that the changes were later in shoots than in roots.It was reasonable to suppose that due to the remobilization of Pi from root to shoot during Pi stress，the shoot was later under Pi stress than the root in tomato，so the changes of proteins in response to Pi stress were later in shoots than in roots.The contents of tonoplast proteins from the roots of tomato under Pi starvation approximated to those of their unstressed controls respectively.However，the components of polypeptides under Pi starvation differ from those of the controls.These results indicated that Pi stress had no effect on the total amount of synthesis of proteins but has some effect on the differential expression of proteins during protein synthesis.SDS-PAGE showed that there were two novel polypeptides (49.9，52.6 kD) that appeared in tonoplast under Pi starvation for 5 day but not in control.Because Pi stress treatment duration in our study was relatively short which had no serious effect on the growth of the seedlings，the differential expression of polypeptides above-mentioned (49.9，52.6 kD) may be specific to Pi stress.

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