|Table of Contents|

Clone and Construction of Plant Expression Vector of Phenylalanine Ammonia Lyase Gene of Pyrus pyrifolia cv. Pingguoli in Yanbian Area(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2013年07期
Page:
106-112
Research Field:
Publishing date:

Info

Title:
Clone and Construction of Plant Expression Vector of Phenylalanine Ammonia Lyase Gene of Pyrus pyrifolia cv. Pingguoli in Yanbian Area
Author(s):
WANG Xu;  QU Bai-hong;  LIU Zhen-kun
Department of Agriculture, Yanbian University, Yanji, Jilin 133002
Keywords:
Pyrus pyrifolia cv. Pingguoli;  Phenylalanine Ammonia Lyase（PAL）;  gene cloning;  plant expression vector
PACS:
S 661.2
DOI:
-
Abstract:
Taking Pyrus pyrifolia cv. Pingguoli in Yanbian as material, based on the nucleic acid sequences of Rosaceae plants logged in Genbank, a pair of PAL primers specific for Pyrus pyrifolia cv. Pingguoli was designed through determination of their conserved region by the alignment of multiple sequences using DNAStar-MegAligan software with the reference to Pyrus communis sequence (GenBank: DQ230992.2), and the PAL gene cloning, sequence analysis and molecular evolution were studied. The results showed that the full-length cDNA sequence of Pyrus pyrifolia cv. Pingguoli PAL gene named PyPAL with registry number of JQ247318.1 was obtained by RT-PCR. Sequence analysis showed that the full-length cDNA of Pyrus pyrifolia cv. Pinguoli PAL was a complete open reading frame of 2 160 bp and speculated to encode 719 amino acids, and it had a homology over 95% with those of Pyrus bretschneideri and Pyrus communis while the homology of the encoded amino acid sequences reached over 95%. And a plant expression vector contained PAL gene recombinant plasmid PBI121-PyPAL was successfully constructed and transformed into agrobacterium tumefaciens LBA4404 forming an engineering strain, laying the foundations for further study on coloring regulatory genes and genetic improvement for fruit coloring of Pyrus pyrifolia cv. Pingguoli.

References:

[1]李跃,刘延吉.果实花青苷代谢机制及调控技术研究[J].安徽农业科学,2007,35(16):4755-4756,4759.

[2]杨林先.套袋苹果梨果实着色与色素及相关酶的研究[D].延吉:延边大学,2010.

[3]李伟.套袋及外源物质处理对苹果梨果实着色影响的研究[D].延吉:延边大学,2011.

[4]徐晓梅,杨署光.苯丙氨酸解氨酶研究进展[J].安徽农业科学,2009,37(31):15115-15119,15122.

[5]张士鸿,金青,樊洪泓,等.砀山酥梨果实苯丙氨酸解氨酶基因cDNA克隆及序列分析[J].激光生物学报,2010,19(1):97-103.

[6]刘志浩,张玉星,申连英,等.鸭梨苯丙氨酸解氨酶基因cDNA片断的克隆与序列分析[C].梨科研与生产进展(五),2011:102-105.

[7]孙百灵,王旭,曲柏宏.苹果梨果实着色相关PAL基因片段克隆及表达分析[J].延边大学农学学报,2012,34(1):1-5.

[8]宗成文.葡萄花发育相关基因的克隆与表达特性研究[D].南京:南京农业大学,2007.

[9]周生茂,王玲平,向珣,等.山药PAL基因全长cDNA序列的克隆、表达与分析[J].核农学报,2008,22(6):781-788.

[10]薛永常.杨树木质素合成酶PAL基因的克隆、鉴定及其表达载体的构建[D].北京:中国林业科学研究院,2001.

[11]黄胤怡.查尔酮合酶基因cDNA的克隆及对茑萝的遗传转化[D].厦门:厦门大学,2002.

[12]曲柏宏,金香兰,陈艳秋,等.苹果梨分类地位的RAPD鉴定[J].吉林农业大学学报,2003,25(3):292-295.

[13]鲁凤娟,张宇星.利用AFLP分子标记鉴定苹果梨的分类地位[J].安徽农业科学,2009,37(5):1937-1938.

[14]吴松权,祖元刚,管清杰,等.膜荚黄芪苯丙氨酸解氨酶基因的克隆与序列分析[J].中草药,2010,41(3):456-460.

[15]史梦远,王海涛,张芳琳.应用生物信息学网络资源分析预测融合蛋白的二级结构及其理化性质[J].中国组织工程研究与临床康复,2008,12(9):1685-1688.

[16]高东尧.高山红景天苯丙氨酸解氨酶基因的克隆及遗传转化[D].长春:吉林大学,2006.

[17]Nakashima J, Awano T, Takabe K, et al. Immunocyto chemical localization of phenylalanine ammonia-lyase and cinnamyl alcohol dehydrogenase in differentiating tracheary elements derived from Zinnia mesophyll cells[J].Plant Cell Physiology,1997,38(2):113-123.


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Last Update: 2014-08-24