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Preliminary Study on Callus Induction of  Corydalis decumbens(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2013年15期
Page:
125-127
Research Field:
Publishing date:

Info

Title:
Preliminary Study on Callus Induction of  Corydalis decumbens
Author(s):
WANG Fei; ZHU Xiao-lan; HE Sha-sha; LIAO Yan; HUANG Sheng-he
Fuzhou Medical College,Nanchang University,Fuzhou,Jiangxi 344000
Keywords:
Corydalis decumbens(Thunb.) Pers.; tissue culture; callus
PACS:
S 567.23+9
DOI:
-
Abstract:
Taking  Corydalis decumbens(Thunb.) Pers.as material,the effect of different disinfection methods,explants and hormone proportion on callus induction were studied.The results indicated that the better disinfection treatment was 10 min with 0.1% HgCl2for blade,but 8 min for tuber and leafstalk.As an explants,leafstalk was superior to tuber and blade.The induction rate (43.3%) was the highest in the MS medium with 0.5 mg/L NAA+1.5 mg/L 6-BA for blade,but the induction rate (80.0%) was the highest if the leafstalk was put in the MS medium with 1.0 mg/L NAA+1.5 mg/L 6-BA.

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Last Update: 2014-08-12