|Table of Contents|

Diversity Analysis of Gene Expression at Dormancy Stage of Lateral Buds in Populus yunnanensis by cDNA-AFLP Markers(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2013年15期
Page:
117-121
Research Field:
Publishing date:

Info

Title:
Diversity Analysis of Gene Expression at Dormancy Stage of Lateral Buds in Populus yunnanensis by cDNA-AFLP Markers
Author(s):
LI Li1; JIANG Tao1; 2; ZHOU An-pei1; ZONG Dan1; ZHAO Yue-ming1; HE Cheng-zhong1; 2; LIU Dong-yu1
1.Key Laboratory for Forest Resources Conservation and Use in the Southwest Mountains of China,Ministry of Education,Southwest Forestry University,Kunming,Yunnan 650224；
2.Key Laboratory of Biodiversity Conservation in Southwest China,State Forestry Administration,Southwest Forestry University,Kunming,Yunnan 650224
Keywords:
lateral buds of Populus yunnanensis; dormancy stage; cDNA-AFLP; genetic differences
PACS:
S 503.53
DOI:
-
Abstract:
To reveal the molecular mechanism of branching characteristic difference in Populus yunnanensis,taking 59 three-year-old seedlings of plus trees clones of P.yunnanensis collected from Sichuan Province and Yunnan Province as the tested materials,cDNA AFLP molecular technique was conducted to examine the diversity of gene expression at dormancy stage of lateral buds of the seedlings.The results showed that a total of 608 bands including 353 different bands were amplified using selected seven primer combinations.Nei’s gene diversity index and Shannon’s information index were 0.1061 and 0.1856 respectively.Gene differentiation coefficience was 0.0167,and gene flow was 29.42,which indicated a lower genetic differentiation existed among populations of P.yunnanensis.Analysis of molecular variance (AMOVA) showed that variance was 0.99% among populations and 99.01% within population.Moreover,the variance only was highly and significantly different among population.The results suggested that gene expression was complicated at dormancy stage of lateral buds.Furthermore,the clustering results showed that most of the clones held the same branching characteristics fell into subcluster unit or single cluster unit.The results of this study laid the foundation of the previous research for further screening and cloning of genes related to branching in P.yunnanensis.

References:

[1]何承忠,张志毅,陈宝昆,等.滇杨遗传改良策略初论[J].西部林业科学,2004,33(1):44-48.
[2]Chandra J P,Ram A.Trial on exotic poplars in Himachal Pradesh[J].Van Vigyan,1973,11(1/2):1-6.
[3]Burfal B S,Meena K L,Sharama R C,et al.Status of poplar in Uttar Pradesh[J].India Forester,2001,127(2):137-143.
[4]李忠洪,张洪清.杨树山地造林品种比较试验[J].贵州林业科技,1995,23(3):17-19.
[5]殷书柏,吕宪国.湿地功能快速评价中的若干理论问题[J].湿地科学,2006,4(1):1-6.
[6]崔丽娟.湿地价值评价研究[M].北京:科学出版社,2001.
[7]欧阳云志,王如松,赵景柱.生态系统服务功能及其生态经济价值评价[J].应用生态学报,1999,10(5):635-640.[8]俞穆青,田卫,孙道玮,等.湿地资源开发环境影响评价探讨[J].东北师范大学学报(自然科学版),2001,32(1):84-89.
[9]王琪,吴磊,伦小文,等.吉林省自然保护区的管理有效性研究[J].吉林化工学院学报,2005,22(1):24-26.
[10]温志宏,黄敏仁.美洲黑杨冠层光截获特性的遗传学研究[J].南京林业大学学报,1992,16(3):11-17.
[11]Shimizu S S,Mori H.Control of outgrowth and dormancy in axillary buds[J].Plant Physiol,2001,127(4):1405-1413.
[12]Becham C,Heven R S,Bruijn Z M,et al.Visualization of differential gene expression using a novel method of RNA fingerprinting based on AFLP：analysis of gene expression during potato tuber development[J].Plant Journal,1996,9(6)：745-753.
[13]Becham C W B,Ronald J F J,Oomen,et al.Transcript imaging with cDNA-AFLP：a step by step protocol[J].Plant Molecular Biology Reporter,1998,16(2):157.
[14]韩斌,彭建营.cDNA-AFLP技术及其在植物基因表达研究中的应用[J].西北植物学报,2006,26(8):17531758.
[15]Bensch S,kesson M.Ten years of AFLP in ecology and evolution：why so few animals[J].Molecular Ecology,2005,14:2899-2914.
[16]Yeh F C,Boyle T J B.Population genetic analysis of codominant and dominantmarkers and quantitative traits[J].Belgian J Bot,1997,129:157.
[17]Cannell M G R,Last F T.熊文愈.树木生理与遗传改良[M].吴贯明,译.北京:中国林业出版社,1980.
[18]Isebrands J G.Genetic variation in phytosynthetic traits among  Populus clones in relation to yield[J].Plant Physiological Biochemistry,1988,26:427-437.
[19]David A K.Tree allometry,leaf size and adult tree size in old growth forests of western Oregon[J].Tree Physiology,1991,9:369-381.
[20]Cline M G.Control mechanisms of sylleptic branching in hybrid  Populus[J].Plant Molecular Biology and Biotechnology PMBB Research Symposium,2000.
[21]Cline M G,Dong il K.A preliminary investigation of the role of Auxin and Cytokinin in sylleptic branching of three hybrid poplar clones exhibiting contrasting degrees of sylleptic branching[J].Annals of Botany,2002,90:417-421.
[22]万雪琴,张帆,胡庭兴.30个杂交杨无性系扦插苗分枝习性研究[J].四川林业科技,2008,29(1):43-46.
[23]夏宁.桃设施栽培几个技术及其原理的研究[D].泰安:山东农业大学,1997.
[24]高升东.桃自然休眠过程中外源激素对花芽碳水化合物的调控效应[J].园艺学报,2002(2):104-107.
[25]陈登文,王飞,高爱琴,等.休眠期间低温积累对杏枝芽生理生化的影响[J].西北植物学报,2000,20(2):212217.
[26]Groot S P C,Keizer L C P,de Ruiter W,et al.Seed and fruit set of the lateral suppressor mutant of tomato[J].Scientia Horticulturae,1994,59:157-162.
[27]Dellagi A,Brich P R J,Heilbronn J.cDNA AFLP analysis of differential gene expression in the prokaryotic plant pathogen  Erwinia carotovora[J].Microbiology,2000,146(1):165-171.
[28]陈荣平,刘磊,万秀清,等.TMV侵染烟草基因差异表达的cDNA-AFLP分析[J].作物学报,2012,38(1):62-70.

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Last Update: 2014-08-12