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Analysis of Genetic Relationship for Punica granatum L.Germplasm Based on Peroxidase Isozyme(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2012年19期
Page:
124-127
Research Field:
Publishing date:

Info

Title:
Analysis of Genetic Relationship for Punica granatum L.Germplasm Based on Peroxidase Isozyme
Author(s):
MA Li; WANG Yu-hai; MING Dong-feng
College of Life Science，Zaozhuang University，Zaozhuang,Shandong 277160
Keywords:
Punica granatum L.(pomegranates); germplasm; POD isozyme; cluster analysis; genetic relationship
PACS:
S 665.4
DOI:
-
Abstract:
The peroxidase (POD) isozyme of 19 pomegranates germplasm cultivars were determined by polyarcylamide gel electrophoresis (PAGE) and the genetic relationship were studied with clustering analysis method.The results showed that a better polymorphism was observed in POD enzyme.The number，the activity and Rf of POD enzyme were difference among 19 pomegranates.12 enzyme bands were detected and there was a common locus for total experiment material in the zymogram patterns；the two specific locuses that Rf was 0.216 and 0.113 were obtained for ‘Daqingpisuan’ cultivar and ‘Damayatian’ cultivar respectively.Furthemore，the clustering analysis indicated 19 pomegranates were divided into five groups based on 0.60 of similarity coefficient，which indicated that the genetic difference between groups was significant.

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Last Update: 2014-09-09