|Table of Contents|

Study on in vitro Regeneration of Zingiber officinale Rosc.by Organogenesis(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2012年19期
Page:
121-123
Research Field:
Publishing date:

Info

Title:
Study on in vitro Regeneration of Zingiber officinale Rosc.by Organogenesis
Author(s):
SUN XiaQI Cui-yingQIN Yan
Institute of Bio-Technology,Laiwu College for Vocational Technology,Laiwu,Shandong 271100
Keywords:
Zingiber officinale Rosc.organogenesisin vitro regeneration
PACS:
S 632.5
DOI:
-
Abstract:
Taking vegetative buds of Zingiber officinale Rosc.as explants,its differentiation with different combinations and concentrations of plant hormone were studied by the method of orthogonal design.The results showed that the aseptic buds were cultured on MS medium supplemented with a combination of 2.0 mg/L KT and 1.0 mg/L NAA gave the shoot and root differentiation,and the multiple shoot showed mean number of 3.5.The repeated subculture resulted in rapid plantlets multiplication during 60 days.It was to provide an effective method for obtaining large scale planting material of ginger by the organogenesis pathway.

References:

[1]Hosoke TSagawa Y.Clonal propagation of ginger (Zingiber officinale?Rosc.) through tissue culture[J].Hort Science,1997,12(5):451-452.

[2]Balachandran S MBhat S RChandel K P S.In vitro?clonal multiplication of turmeric (Curcuma?spp.) and ginger (Zingiber officinale?Rosc.) [J].Plant Cell Reports,1990(8):521-524.

[3]Dekkers A JRao A NGoh C J.In vitro?storage of multiple shoot cultures of ginger at ambient temperature of 24~29[J].Scientia Horticulturae,1991,47:157-167.

[4]Villamor C.Influence of media strength and sources of nitrogen on micropropagation of ginger,?Zingiber officinale?Rosc [J].E-International Scientific Research Journal,2010,2(2):150-155.

[5]Malamug J J FInden AAsahira T.Plantlet regeneration and propagation from ginger callus [J].Scientia Horticulturae,1991,48:89-97.

[6]Babu K NSamasudeen KRatnambal M J.In vitro?plant regeneration from leaf-derived callus in ginger [J].Plant Cell Tissue and Organ Culture,1992,29:71-74.

[7]Kackar ABhat S RChandel K P S,et al.Plant regeneration via somatic embryogenesis in ginger [J].Plant Cell Tissue and Organ Culture,1993,32:289-292.

[8]Sultana AHassan LAhmad S Det al.In vitro?regeneration of ginger using leafshoot tip and root explants [J].Pakistan Journal of Botany,2009,41(4):1667-1676.

[9]Kavyashree R.An efficient in vitro protocol for clonal multiplication of Ginger-var.varada[J].Indian Journal Biotechnology,2009(8):328-331.

[10]Yunus M FAziz M AKadir M Aet al.In vitro?propagation of Etlingera elatior?(Jack) (torch ginger)[J].Scientia Horticulturae,2012,135:145-150.

[11]BhagyalakshmiSingh N S.Meristem culture and micropropagation of a variety of ginger (Zingiber officinale?Rosc.) with a high yield of oleoresin[J].Journal of Horticultural Science,1988,63(2):321-327.

[12]李红梅,张侠,宋莉璐,.生姜茎尖的组织培养及试管苗快繁体系的研究[J].山东科学,2008,21(5):36-38.

[13]缪静,柏新富.姜茎尖的离体培养与试管苗快繁[J].中草药,2004,35(10):1178-1180.

Memo

Memo:
-
Last Update: 2014-09-09