|Table of Contents|

Sequencing and Analysis of the Genome of Lily mottle virus (LMoV) Jingyu Isolate

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2023年24
Page:
59-64
Research Field:
Publishing date:

Info

Title:
Sequencing and Analysis of the Genome of Lily mottle virus (LMoV) Jingyu Isolate
Author(s):
ZHANG Chunyu1; LI Xiaoyu1; WANG Chen1; HUANG Ruixian2; HUANG Shumin2; WANG Yongzhi1
(1.Institute of Plant Protection，Jilin Academy of Agricultural Sciences（Northeast Agricultural Research Center of China），Gongzhuling，Jilin 136100；2.Jingyu Agricultural Technology Extension Center,Baishan，Jilin 135200)
Keywords:
Lily mottle virus（LMoV）; genome
PACS:
S 435.29
DOI:
10.11937/bfyy.20232243
Abstract:
The infected leaves of wild lily were taken from Jingyu county，Baishan city，Jilin Province.Lily mottle virus (LMoV) was detected by small RNA deep sequencing and the whole genome of LMoV Baishan-Jingyu isolate (LMOV-JY) was determined and analyzed by segmentation cloning method，in order to provide reference for detection，prevention and control of lily virus disease in Jilin Province.The results showed that the genome of LMoV-JY consisted of 9 648 nucleotides (nt) (Genbank Accession No.MT795719).A large open reading frame (ORF) located at nt positions from 154 to 9 444 which encoding a polypeptide of 351.46 kD.By compairing with other isolates of LMoV，the nucleotide identity was from 82.82% to 97.85%，and the amino acid identity was from 92.96% to 98.64%，and it shared the highest identity with the Dalian isolate LMoV-DL (HM222521).Amino acid sequences comparison of the CP gene of LMoV-JY with other 54 isolates in GenBank showed that all of the isolates could be divided into two subgroups.

References:

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Last Update: 2024-01-09