|Table of Contents|

Flowering Induction of F1 Generation of Cymbidium hybridium‘Dafeng’×C.serratum var.goeringii ‘Taijishengmei’ in vitro

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2023年24
Page:
52-58
Research Field:
Publishing date:

Info

Title:
Flowering Induction of F1 Generation of Cymbidium hybridium‘Dafeng’×C.serratum var.goeringii ‘Taijishengmei’ in vitro
Author(s):
ZHANG Zichen1LI Zhilin1HE Fengmei1QU Suping2LI Yefang1WANG Yuying1
(1.College of Horticulture and Landscape,Yunnan Agricultural University,Kunming,Yunnan 650201;2.Flower Research Institute,Yunnan Academy of Agricultural Sciences,Kunming,Yunnan 650000)
Keywords:
F1 generation of Cymbidium hybridium ‘Dafeng’×C.serratum var.goeringii ‘Taijishengmei’plantletplant growth regulatorflower bud formationflowering in vitro
PACS:
S 682.1+9
DOI:
10.11937/bfyy.20232482
Abstract:
The F1 generation plantlets of Cymbidium hybridium ‘Dafeng’ × C.serratum var.goeringii ‘Taijishengmei’ were used as test material pretreated with PP333 or CCC,and subsequently added with different concentrations of 6-BA to determine plant morphology and physiological indicators,a test-tube flowering induction system was established and the childhood period of orchid plants was shortened,in order to provide reference for breeding of orchid.The results demonstrated that pretreatment with different concentrations of PP333 or CCC inhibited the increasing of plant height,root number and fresh weight,but promoted leaf division.The test tube seedlings were pre-cultured in 1/2MS+PP333 1.0 mg·L-1+banana puree 120 g·L-1+sucrose 30 g·L-1+agar 8 g·L-1+activated carbon 0.5 g·L-1 for 30 days.Then,the culture medium of 1/2MS+NAA 0.2 mg·L-1+banana pulp 120 g·L-1+sucrose 30 g·L-1+agar 8 g·L-1+activated carbon 0.5 g·L-1 could induce the flower bud flowering,the flower bud induction rate was 6.67%,but the flower bud did not open successfully in the late stage.The contents of soluble sugar and soluble protein were higher than CK under PP333 or CCC pretreatment,which promoted the increase of C/N value and the decrease of total nitrogen content.

References:

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Last Update: 2024-01-09