|Table of Contents|

Detection and Analysis of Strawberry Mild Yellow Edge Virus in Different Varieties of Strawberry Seedlings in Beijing Area

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2024年1
Page:
38-44
Research Field:
Publishing date:

Info

Title:
Detection and Analysis of Strawberry Mild Yellow Edge Virus in Different Varieties of Strawberry Seedlings in Beijing Area
Author(s):
ZHANG Jiaxing1; WANG Qiushi1; ZHOU Yu2; HUANG Yaqi1; YAN Teng1; SHANG Qiaoxia1
(1.College of Bioscience and Resource Environment,Beijing University of Agriculture/Key Laboratory for Northern Urban Agriculture of Ministry of Agriculture and Rural Affairs,Beijing 102206;2.Agricultural Technology Extension Station of Changping District in Beijing，Beijing 102200)
Keywords:
strawberry mild yellow edge virus; different varieties; RT-PCR; disease investigation
PACS:
S 436.68
DOI:
10.11937/bfyy.20231977
Abstract:
The effects of strawberry mild yellow edge virus (SMYEV) on different varieties of strawberry seedlings were studied by field investigation and RT-PCR using 36 varieties of strawberry seedlings from Beijing area as materials,in order to provide reference for the breeding and popularization of strawberry varieties.The results showed that among the 431 strawberry seedlings collected，30 SMYEV positive samples were detected，and the virus detection rate was 6.96%.SMYEV was detected from 8 varieties of strawberry seedlings，and the detection rate was 6.5%-23.1%，while no SMYEV were found in the other 28 varieties.The nucleotide sequences of SMYEV coat protein (CP) of three Beijing isolates were obtained by gene cloning，sequencing and alignment analysis.The homology of CP gene sequence between Beijing isolate BJHY253 and American isolate WSU1988 was 98.63%.The homology of CP gene sequence between Beijing isolate BJHYZ83 and Argentina isolate Berra-2 was 99.04%.The homology of CP gene sequence between Beijing isolate BJLYN and Fujian isolate FJ2 was 98.63%.In this study，SMYEV in different strawberry varieties was detected and sequenced.It was found that the detection rate of SMYEV in various strawberry varieties was different.The SMYEV sequences in strawberry seedlings in Beijing were diverse and belonged to different strains.These results will provide data support for field production and virus prevention and control of strawberry.

References:

［1］XIANG Y,NIE X,BERNARFY M,et al.Genetic diversity of strawberry mild yellow edge virus from eastern Canada［J］.Archives of Virology,2020,165(4):923-935.［2］LAMPRECHT S,JELKMANN W.Infectious cDNA clone used to identify strawberry mild yellow edge-associated potexvirus as causal agent of the diseas［J］.Journal of General Virology,1997,78(9):2347-2353.［3］王建辉,刘建军,陈克玲,等.草莓轻型黄边病毒检测研究［J］.西南农业学报,2016,29(12):2866-2870.［4］KING A M,LEFKOWITZ E,ADAMS M J,et al.Virus taxonomy:Ninth report of the international committee on taxonomy of viruses［M］.Holand:Elsevier Academic Press,2011.［5］THOMPSON J R,JELKMANN W.Strain diversity and conserved genome elements in strawberry mild yellow edge virus［J］.Archives of Virology,2004,149(10):1897-1909.［6］尚巧霞.介体传播的草莓病毒研究进展［J］.植物保护学报,2015,42(4):488-496.［7］江山,陈道,叶立乐,等.草莓轻型黄边病毒中国分离物全基因组序列测定和分析［J］.病毒学报,2022,38(5):1187-1194.［8］陈柳,冉策,尚巧霞,等.草莓轻型黄边病毒北京分离物的CP基因序列分析［J］.中国农学通报,2015,31(3):229-233.［9］陈晓军，王敬东，马洪爱，等.利用RT-PCR对脱毒草莓苗病毒检测研究［J］.北方园艺,2010(23):146-148.［10］王鹏,王月,王文晓,等.我国草莓新品种权保护现状与分析［J］.中国果树,2023(4):129-135.［11］杜茜,张学明,张正坤,等.公主岭霉素对草莓生长及果实品质的影响［J］.北方园艺,2021(5):42-47.［12］常琳琳,董静,钟传飞,等.中国育成草莓品种的系谱分析［J］.果树学报,2018,35(2):158-167.［13］徐艺格,王丽娟.草莓品质育种研究进展［J］.北方园艺,2020(18):152-157.［14］杨爽,李海鹏,杨培鑑,等.不同草莓品种对白粉病的抗性调查［J］.北方园艺,2014(10):104-106.［15］马燕会,齐永志,赵绪生,等.自毒物质胁迫下不同草莓品种枯萎病抗性变化的研究［J］.河北农业大学学报,2012,35(2):93-97.［16］贾薇,梁中午,张瑶瑶,等.兼抗白粉病和灰霉病草莓品种的筛选及田间抗性评价［J］.江苏农业科学,2019,47(14):132-135.［17］周宇.草莓上三种病毒的实时定量检测与草莓镶脉病毒全序列测定及分析［D］.北京:北京农学院,2020.［18］陈柳,邢冬梅,刘正雄,等.京郊设施蔬菜黄瓜花叶病毒的检测［J］.中国农学通报,2015,31(20):82-85.［19］邹文超，沈林林，沈建国，等.马铃薯Y病毒多基因系统发育分析及其在株系鉴定中的应用［J］.遗传,2017,39(10):918-929.［20］陈莹,田艳珍,王芳,等.浙江省马铃薯病毒病检测分析［J］.植物保护,2020,46(5):110-115,121.［21］李金岭,罗晶,牛小义,等.陕西省烟草主要病毒病种类及植物带毒的检测［J］.西北农林科技大学学报(自然科学版),2013,41(1):78-84.［22］杨菊梅,何永红,马建忠,等.鸡卵黄抗体技术在5种草莓病毒快速检测中的应用［J］.兰州大学学报(自然科学版)，2021,57(3):353-359,368.［23］王佳,崔高峰,祝宁,等.北京地区设施草莓8种病毒的酶联免疫吸附测定检测［J］.微生物学通报,2021,48(3):1048-1056.［24］王佳,白莹雪,祝宁,等.基于小RNA深度测序技术鉴定侵染我国部分省市草莓种苗的病毒种类［J］.植物保护,2022,48(3):225-232,241.［25］何成勇,高德航,范灵姣,等.利用小RNA深度测序快速鉴定草莓病毒［J］.中国农业大学学报,2021,26(8):54-60.［26］朱海生,花秀凤,陈敏氡,等.四种草莓病毒SMoV、SVBV、SCV、SMYEV多重RT-PCR检测［J］.核农学报,2013,27(11):1630-1635.［27］何秉青,祝宁,齐长红,等.不同草莓品种对3种病虫害的抗性及杀虫剂对二斑叶螨的防效评价［J］.中国蔬菜,2023(3):98-103.［28］王佳.我国部分省市草莓种苗病毒种类检测与序列分析［D］.北京:北京农学院,2021.［29］杨洪一,代红艳,李丽丽,等.草莓轻型黄边病毒3′末端序列多态性研究［J］.植物病理学报,2008(1):96-99.［30］王燕飞,玉山江·麦麦提,孙柳清,等.南瓜蚜传黄化病毒遗传多样性分析［J］.植物病理学报,2021,51(3):351-358.［31］何欢乐,阳静,蔡润,等.草莓茎尖培养脱毒效果研究［J］.北方园艺,2005(5):79-81.［32］邱静.草莓茎尖超低温疗法脱毒技术体系的建立［D］.雅安:四川农业大学,2015.

Memo

Memo:
-
Last Update: 2024-01-22